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A method for collecting and preserving dormant eggs of black pearl killifish

A preservation method and technology of dormant eggs, applied in fish farming, application, climate change adaptation, etc., can solve the problems of inconvenient inspection of developmental status, low hatching rate, and high labor cost, and achieve convenient development inspection, easy screening and separation, The effect of speeding up the filter time

Active Publication Date: 2020-11-06
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Medaka and black pearl killifish are classified as two types of fish in different families and genera. The former lays its eggs and hangs them outside the cloaca of the mother through sticky silk, while the latter lays its eggs in the soft underwater soil Among them, the method cannot be generalized, and this method is very time-consuming and laborious, and it is impossible to select thoroughly, so it is not suitable for commercial operation
[0005] In the latter method, it is inconvenient to check the development status because the eggs are mixed in the soil, and the hatching time is difficult to be accurate, resulting in a low hatching rate, and repeated operations such as collecting unhatched eggs, drying them, sealing them for storage, and launching them again for hatching are required.
The work is cumbersome, the labor cost is high, the hatching rate is low, and it is difficult to meet the needs of commercial production
In addition, eggs and peat soil are mixed together, which is difficult to disinfect, resulting in the occurrence of diseases such as dinoflagellates during the growth of larvae in the later stage. In addition, eggs and soil are mixed with water to hatch, resulting in a small number of larvae that have been removed from the membrane. And die in it, and when it is recovered together with unhatched eggs and stored in dormancy again, it will rot and mold and affect other unhatched eggs

Method used

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  • A method for collecting and preserving dormant eggs of black pearl killifish
  • A method for collecting and preserving dormant eggs of black pearl killifish
  • A method for collecting and preserving dormant eggs of black pearl killifish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Oviposition substrate preparation: excavate pond mud, add tap water, stir, settle, remove duckweed and other floating matter, wrap it with 100-mesh nylon sieve, squeeze and sieve, remove snails, chironomus larvae and other benthic organisms and impurities , spread out to a thickness of 15mm, and dry in an oven at 120°C for 30min. Purchase commercially available ADA mud, pulverize it in a blender, filter through a 40-mesh nylon sieve, and pulverize what fails to filter. It is obtained by mixing 70 wt% of the treated pond mud and the rest of the ADA mud powder.

[0034] Preparation of antibacterial solution: Dissolve methylene blue in peat soil leaching solution, the concentration of methylene blue is 5ppm. The peat soil leaching solution is prepared by soaking the peat soil in water at a mass ratio of 1:2 for one month.

[0035] Collection, preservation and incubation of dormant eggs: Lift the 20-mesh semi-rigid screen laid on the bottom of the spawning tank in advance...

Embodiment 2

[0038] Oviposition substrate preparation: excavate pond mud, add tap water, stir, settle, remove duckweed and other floating matter, wrap it with 100-mesh nylon sieve, squeeze and sieve, remove snails, chironomus larvae and other benthic organisms and impurities , spread out to a thickness of 15mm, and dry in an oven at 120°C for 30min. Purchase commercially available ADA mud, pulverize it in a blender, filter through a 40-mesh nylon sieve, and pulverize what fails to filter. It is obtained by mixing 65 wt% of the treated pond mud and the rest of the ADA mud powder.

[0039]Preparation of antibacterial solution: Dissolve methylene blue in peat soil leaching solution, the concentration of methylene blue is 6ppm. The peat soil leaching solution is prepared by soaking the peat soil in water at a mass ratio of 1:2 for 40 days.

[0040] Collection, preservation and incubation of dormant eggs: Lift the 20-mesh semi-rigid screen laid on the bottom of the spawning tank in advance, s...

Embodiment 3

[0043] Oviposition substrate preparation: excavate pond mud, add tap water, stir, settle, remove duckweed and other floating matter, wrap it with 100-mesh nylon sieve, squeeze and sieve, remove snails, chironomus larvae and other benthic organisms and impurities , spread out to a thickness of 15mm, and dry in an oven at 120°C for 30min. Purchase commercially available ADA mud, pulverize it in a blender, filter through a 40-mesh nylon sieve, and pulverize what fails to filter. It is obtained by mixing 75wt% of the treated pond mud and the rest of the ADA mud powder.

[0044] Preparation of antibacterial solution: Dissolve methylene blue in peat soil leaching solution, the concentration of methylene blue is 6ppm. The peat soil leaching solution is prepared by soaking the peat soil in water at a mass ratio of 1:2 for 20 days.

[0045] Collection, preservation and incubation of dormant eggs: Lift the 20-mesh semi-rigid screen laid on the bottom of the spawning tank in advance, s...

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PUM

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Abstract

The invention relates to a collection and storage method for fish eggs, and discloses a collection and storage method for dormant eggs of cynolebias nigripinnis. The method comprises the following steps: (1) egg collection; (2) washing; (3) previewing; (4) soaking; (5) packaging; (6) storage of dormant eggs. By preparing a special egg-laying medium, the invention designs a corresponding filter screen, consequently, great workload caused by the work of egg selection in the traditional reproduction method is solved, and manpower is saved by 90 percent or more; moreover, an egg-carrying materialis utilized to solve the adverse influence caused by peat soil storage in the traditional reproduction method, and thereby technical support is provided for the large-scale reproduction of the aquarium fish.

Description

technical field [0001] The invention relates to a method for collecting and preserving fish eggs, in particular to a method for collecting and preserving dormant eggs of black pearl killifish. Background technique [0002] The black pearl killifish is an annual oviparous killifish in South America. It has a very attractive body color. It can be said to be one of the few fish species in freshwater ornamental fish that can be compared with sea coral reef fish. In addition to their small size, they are lively With an active personality, it is a perfect match in a planted tank. In addition, most oviparous killifish have wide adaptability to water quality and are easy to raise. These characteristics make them one of the most popular tropical ornamental fish species abroad. [0003] When the black pearl killifish breeds, usually sexually mature male and female broodstock are selected and raised separately in a breeding tank. A small flower pot with peat soil is placed in this tan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/17
CPCA01K61/17Y02A40/81
Inventor 施振宁
Owner ZHEJIANG NORMAL UNIVERSITY
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