Method for extracting and enriching radix curcumae longae

A turmeric and enrichment technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., can solve problems such as serious environmental pollution, large sample dosage, and high solvent toxicity, and achieve a wide range of applications and operating environments. Safe and reliable, the effect of improved sensitivity

Inactive Publication Date: 2018-09-14
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such traditional extraction methods have disadvantages such as time-consuming, highly

Method used

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  • Method for extracting and enriching radix curcumae longae
  • Method for extracting and enriching radix curcumae longae
  • Method for extracting and enriching radix curcumae longae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1. Investigate the impact of the type of ionic liquid on the enrichment effect

[0053] 1.1 Take 6 clean 30mL stoppered jars, numbered 1, 2, 3, 4, 5, 6, add 0.5mL of 20mg / mL turmeric ionic liquid extract, 0.02mL of 200μg / mL chitosan nanoparticles and remove Ionized water 9.48mL. The turmeric ionic liquid extract added on No. 1 is extracted from 1-ethyl-3-methylimidazole-L-lactate, and the turmeric ionic liquid extract added on No. 2 is 1-dodecyl-3-methyl Imidazolium mesylate was extracted, the turmeric ionic liquid extract added on No. 3 was extracted from 1-hexylpyridine tetrafluoroborate, and the turmeric ionic liquid extract added on No. 4 was 1-dodecyl-3 chloride -Methylimidazole, the turmeric ionic liquid extract added on No. 5 is extracted from 1-dodecyl-3-methylimidazole nitrate, and the turmeric ionic liquid extract added on No. 6 is 1-dodecyl bromide Alkyl-3-methylimidazolidine.

[0054] 1.2. Put the jar containing the mixture into the orbital shaker...

Embodiment 2

[0065] Embodiment 2. investigate the impact of ionic liquid concentration on enrichment effect

[0066] 2.1 Take 5 clean 30mL stoppered jars, numbered 1, 2, 3, 4, 5, add 0.5mL of 20mg / mL turmeric ionic liquid extract, 0.02mL of 200μg / mL chitosan nanoparticles and deionized water 9.48mL. The turmeric ionic liquid extract added on the 1st is 100mM bromide 1-dodecyl-3-methylimidazolium, and the turmeric ionic liquid extract added on the 2nd is 150mM bromide 1-dodecyl-3- The turmeric ionic liquid extract added on the 3rd is 200mM bromide 1-dodecyl-3-methyl imidazole, and the turmeric ionic liquid extract added on the 4th is 250mM bromide 1-decyl Dialkyl-3-methylimidazolium, the turmeric ionic liquid extract added in No. 5 is 300mM bromide 1-dodecyl-3-methylimidazolium.

[0067] 2.2 Put the jar containing the mixture into the orbital shaker and shake for 60s. Draw up the solution in the jar with a 10 mL disposable syringe.

[0068] 2.3 Remove the needle, install a 0.22 μm filte...

Embodiment 3

[0078] Embodiment 3. investigate the impact of chitosan nanoparticle concentration on enrichment effect

[0079] 3.1 Take 6 clean 30mL jars with stoppers, numbered 1, 2, 3, 4, 5, 6, add 0.5mL of 20mg / mL turmeric ionic liquid extract to each, and add 200μg / mL chitosan nanoparticles to No. 1 Dispersion solution 0μL and deionized water 9.5mL, No. 2, add 200μg / mL chitosan nanoparticle dispersion 10μL and deionized water 9.49mL, No. 3, add 200μg / mL chitosan nanoparticle dispersion 20μL and deionized Water 9.48mL, add 200μg / mL chitosan nanoparticle dispersion 30μL and deionized water 9.47mL to No. 4, add 200μg / mL chitosan nanoparticle dispersion 40μL and deionized water 9.46mL to No. 6 Add 50 μL of 200 μg / mL chitosan nanoparticle dispersion and 9.45 mL of deionized water, so that the concentrations of chitosan in No. 1-6 mixed solution are: 0.0 μg / mL, 0.2 μg / mL, 0.4 μg / mL , 0.6 μg / mL, 0.8 μg / mL, 1.0 μg / mL.

[0080] 3.2 Put the jar containing the mixture into the orbital shaker and...

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Abstract

The invention discloses a method for extracting and enriching radix curcumae longae, which belongs to the technical field of chemical extraction of natural drugs. The method for extracting and enriching the radix curcumae longae comprises the following steps: 1, preparing chitosan nano particles; 2, crushing medicinal materials; 3, preparing ion liquid and chitosan nano-particle solution wherein the ion liquid is bromo-dodecyl-3-methylimidazole, 200mM, and the chitosan nano-particle is 200 mu g/mL; 4, ultrasonically extracting the ion liquid: adding 0.2 g radix curcumae longae powder into 10mLof the ion liquid in an ultrasonic instrument, and ultrasonically extracting for 30 min at 40 DEG C; 5, enriching chitosan nano-particles: enriching the 1.0mg/mL radix curcumae longae ion liquid extract solution by using 0.6 mu g/mL chitosan nano-particles; and 6, eluting solvent: acetone. By adopting the method, the use of organic solvent is greatly reduced, the pollution and harm of the extraction process can be significantly reduced, the extraction effect and the extraction efficiency are improved, and the method is simple, wide in application range, easy in use and significant in enrichment effect.

Description

technical field [0001] The invention belongs to the field of extraction and preparation of natural medicines. It relates to a method for extracting and enriching traditional Chinese medicines. Specifically, it involves using ionic liquids as extraction solvents and chitosan nanoparticles as adsorbents to extract and enrich active ingredients in the traditional Chinese medicine turmeric. That is, the present invention provides a new method, which skillfully combines the properties of ionic liquid and chitosan nanoparticles, and can quickly and effectively enrich the active ingredients in natural products. Background technique [0002] Natural products are non-synthetic biologically active compounds with rich sources, which have unique pharmacological effects and reliable therapeutic effects. They are mainly derived from chemical constituents in plants, animals, marine organisms, insects, microorganisms and fungi, and also form the basis of important synthetic or semi-synthet...

Claims

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Application Information

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IPC IPC(8): A61K36/9066
CPCA61K36/9066A61K2236/331A61K2236/51A61K2236/55
Inventor 曹君胡雨涵王秋燕
Owner HANGZHOU NORMAL UNIVERSITY
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