A kind of SERS chip and preparation method thereof

A chip and pit technology, which is applied in the field of SERS chip and its preparation, can solve the problems of cumbersome hydrochloric acid cleaning operation, high cost, and difficulty in large-scale preparation, and achieve enhanced SERS effect, better uniformity, and good repeatability Effect

Active Publication Date: 2020-03-24
苏州英菲尼纳米科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is cumbersome to transfer the template and wash with hydrochloric acid, and it is difficult to transfer AAO to other templates to achieve large-scale preparation, and the cost is high

Method used

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  • A kind of SERS chip and preparation method thereof
  • A kind of SERS chip and preparation method thereof
  • A kind of SERS chip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Preparation of silver nano-sol:

[0063] Mix 0.5ml of 30mg / mL silver nitrate aqueous solution with 83ml of ultrapure water, put it in a 100ml three-necked bottle, put it in a water bath and heat to boiling. Afterwards, 1.5 ml of trisodium citrate aqueous solution was quickly injected. Stirring was continued for 30 min. The particle size of the obtained silver sol is about 20nm.

[0064] The template (pore diameter 70nm, pore depth 40nm, honeycomb configuration) prepared by nanoimprinting was 5cm*5cm. Soak in the above-mentioned Ag sol for 3 hours, take it out, rinse with water, and blow dry with nitrogen.

[0065] In situ growth of substrates:

[0066] The above-mentioned SERS template loaded with nanoparticles was placed at the bottom of the beaker, and 20 ml of 0.01% chloroauric acid aqueous solution was added. Add 1ml of 1% sodium citrate solution, and then slowly add 3 times molar amount of hydroxylamine hydrochloride solution dropwise under the condition of me...

Embodiment 2

[0068] Preparation of silver nano-sol:

[0069] Mix 0.5ml of 30mg / mL silver nitrate aqueous solution with 83ml of ultrapure water, put it in a 100ml three-necked bottle, put it in a water bath and heat to boiling. Afterwards, 1.2 ml of trisodium citrate aqueous solution was quickly injected. Stirring was continued for 30 min. The particle size of the obtained silver sol is about 30nm.

[0070] The template (pore diameter 70nm, pore depth 40nm, honeycomb configuration) prepared by nanoimprinting was 5cm*5cm. Soak in the above-mentioned Ag sol for 3 hours, take it out, rinse with water, and blow dry with nitrogen.

[0071] In situ growth of substrates:

[0072] The above-mentioned SERS template loaded with nanoparticles was placed at the bottom of the beaker, and 20 ml of 0.01% silver nitrate aqueous solution was added. Add 0.05 ml of ammonia water, and then slowly add an equimolar amount of formaldehyde solution dropwise under the condition of mechanical stirring. After t...

Embodiment 3

[0074] Preparation of gold nanosol:

[0075] Mix 1ml of 10mg / mL chloroauric acid aqueous solution with 99ml of ultrapure water and put it in a 100ml three-necked bottle, put it in a water bath and heat to boiling. Then inject 2 ml of trisodium citrate aqueous solution quickly. Stirring was continued for 30 min. The particle size of the obtained gold sol is about 35nm.

[0076] The template prepared by laser etching (pore diameter 80nm, pore depth 60nm, honeycomb configuration) 5cm*5cm was placed on a spin coater. Take the concentrated above-mentioned Au sol and spread it on the template, and slowly rotate it at 500 revolutions per minute with an acceleration of 20 revolutions per second for 8 minutes. Remove the spare.

[0077] In situ growth of substrates:

[0078] The above-mentioned SERS template loaded with nanoparticles was placed at the bottom of the beaker, and 20 ml of 0.01% chloroauric acid aqueous solution was added. Add 1 ml of 1% sodium citrate solution, and ...

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Abstract

The invention provides an SERS chip and a preparation method thereof. The SERS chip comprises a substrate, whose surface is provided with a plurality of pits, and nano structural units arranged in thepits. Each nano structural unit comprises a first part in a corresponding pit and a second part out of the pit. At first, first nano particles grow from the pits of the substrate, then second nano particles grow from the positions of the first nano particles, and then the second nano particles stretch out of the pits to prepare the chip. The preparation technology is simple, the efficiency is high, and the cost is low. High performance SERS chips can be massively produced. The commercial needs can be well satisfied. The prepared SERS chip has the advantages of high repeatability, uniform hotspots, stable proprieties, large growth area, and high sensitivity, can be repeatedly used for many times, has a reduced cost, and can be used to detect multiple organic substances with a low concentration.

Description

technical field [0001] The invention relates to Surface-Enhanced Raman Scattering (SERS) technology, in particular to a SERS chip and a preparation method thereof. Background technique [0002] Raman spectroscopy is a kind of scattering spectrum. Raman spectroscopy is based on the Raman scattering effect discovered by Indian scientist C.V. Raman (Raman). It analyzes the scattering spectrum different from the incident light frequency to obtain molecular vibration and rotation. information and is an analytical method applied to the study of molecular structures. This technology is widely used in various fields such as chemistry, physics, biology and medicine because of its fast, simple, repeatable and non-destructive qualitative and quantitative analysis advantages. It is used in pure qualitative analysis, highly quantitative analysis and determination of molecular structure. It also shows unique advantages. SERS enhancement sources include noble metal sols and enhancement s...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): G01N21/65G01N21/01
CPCG01N21/01G01N21/658
Inventor孙海龙郭清华
Owner苏州英菲尼纳米科技有限公司