Lactic acid bacteria vacuum freeze-drying protective agent and freeze-drying technology thereof
A technology of vacuum freeze-drying and lactic acid bacteria, which is applied in the direction of bacteria and preserved microorganisms, can solve the problems of long freeze-drying time and high production cost, achieve good protection, strong general performance, and save freeze-drying time
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Embodiment 1
[0020] The vacuum freeze-drying protective agent for lactic acid bacteria includes 5% glycerin, 14% trehalose, 14% skimmed milk powder, 1.8% vitamin C, 20% gum arabic, and the balance is pure water.
[0021] The freeze-drying method of lactic acid bacteria vacuum freeze-drying protective agent comprises the following steps:
[0022] S1, the lactic acid bacteria vacuum freeze-dried protective agent is sterilized at 121°C for 20 minutes, and placed at room temperature;
[0023] S2, adding the lactic acid bacteria vacuum freeze-drying protective agent placed at room temperature in S1 to the bacteria sludge collected by centrifugation of the fermentation broth according to the addition amount of 20%;
[0024] S3, uniformly mixing the lactic acid bacteria vacuum freeze-drying protective agent with the bacteria sludge collected by centrifugation from the fermentation broth, and fully stirring to emulsify;
[0025] S4, adding the emulsified mixture in S3 into the freeze-drying tray,...
Embodiment 2
[0032] The vacuum freeze-drying protective agent for lactic acid bacteria includes 1% glycerin, 20% trehalose, 5% skimmed milk powder, 3% vitamin C, 10% gum arabic, and the balance is pure water.
[0033] The freeze-drying method of lactic acid bacteria vacuum freeze-drying protective agent comprises the following steps:
[0034] S1, the lactic acid bacteria vacuum freeze-dried protective agent is sterilized at 121°C for 20 minutes, and placed at room temperature;
[0035] S2, adding the lactic acid bacteria vacuum freeze-drying protective agent placed at room temperature in S1 to the bacteria sludge collected by centrifugation of the fermentation broth according to the addition amount of 10%;
[0036] S3, uniformly mixing the lactic acid bacteria vacuum freeze-drying protective agent with the bacteria sludge collected by centrifugation from the fermentation broth, and fully stirring to emulsify;
[0037] S4, add the emulsified mixture in S3 into the freeze-drying tray, and t...
Embodiment 3
[0043] The vacuum freeze-drying protective agent for lactic acid bacteria includes 10% glycerin, 5% trehalose, 20% skimmed milk powder, 0.5% vitamin C, 30% gum arabic, and the balance is pure water.
[0044] The freeze-drying method of lactic acid bacteria vacuum freeze-drying protective agent comprises the following steps:
[0045] S1, the lactic acid bacteria vacuum freeze-dried protective agent is sterilized at 121°C for 20 minutes, and placed at room temperature;
[0046] S2, adding the lactic acid bacteria vacuum freeze-drying protective agent placed at room temperature in S1 to the bacteria sludge collected by centrifugation of the fermentation broth according to the addition amount of 30%;
[0047] S3, uniformly mixing the lactic acid bacteria vacuum freeze-drying protective agent with the bacteria sludge collected by centrifugation from the fermentation broth, and fully stirring to emulsify;
[0048] S4, adding the emulsified mixture in S3 into the freeze-drying tray,...
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