Method for serum-free domestication and culture of human mesenchymal stem cells

Abstract

The invention discloses a method for serum-free domestication and culture of human mesenchymal stem cells. According to the method, human placenta-derived mesenchymal stem cells are allowed to adapt to an in-vitro serum-free culture system, and cell density and cell viability similar to the cell density and cell viability of serum culture are obtained; and compared with conventional culture methods for human mesenchymal stem cells, the method provided by the invention is more economical and effective. With the method, the expression levels of bFGF, PDGF and VEGF associated with blood vessel growth are increased compared with undomesticated cells. The domesticated cells can be prepared for preparing stem cell preparations for intravenous injection; and active factor components secreted by the domesticated cells can be used as components of a mask, an eye mask and a hair mask.

Description

technical field

[0001] The present invention relates to a culture method of human mesenchymal stem cells, and more specifically, the present invention relates to a serum-free in vitro culture of human mesenchymal stem cells by using a unique and safe acclimation culture method. Background technique

[0002] Stem cells are the ancestors of human cells, and all cells in our body are derived from stem cells. When cells in the body age, die or become damaged and degenerate, stem cells grow and transform into cells that can replace them. As seed cells, it is mainly used clinically to treat various refractory diseases of tissue cells and organ damage that cannot be naturally repaired by the body; as immune regulatory cells, it is used to treat immune rejection and autoimmune diseases. Human mesenchymal stem cells (human mesenchymal stem cells, HMSC) is an important member of the stem cell family, derived from the mesoderm in the early development, belonging to pluripotent stem ce...