A kind of cell penetrating peptide and its preparation method and application
A technology of membrane-penetrating peptides and cell membranes, applied in the field of biomedicine, can solve problems such as no unified conclusion, and achieve the effects of low immunogenicity, high membrane-penetrating efficiency, and remarkable membrane-penetrating effect
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Embodiment 1
[0047] By searching the human protein database, the inventors found that the C-terminus of the human SPOCK2 protein (SPARC / osteonectin, CWCV, and Kazal-like domains proteoglycan 2) is tightly bound to the cell membrane, and has multiple polysaccharide adhesion sites, and found that the human Y-like chromosome-encoded testis-specific protein gene 2 (Testis-specific protein Y encoded-like, TSPYL2) encodes a class of protein that participates in nucleosome assembly and maintains cell viability. This protein needs to be combined with DNA to function.
[0048] Further analysis found that a 17-amino acid short peptide (RRRRRKQARRPRRRRAR) at the C-terminal of the SPOCK2 protein was rich in arginine and had a strong positive charge. It was speculated that this short peptide might be a new type of human-derived membrane-penetrating peptide with Autonomous membrane-penetrating function, thus providing the human-derived cell-penetrating peptide (SPOCK2 for short) in this embodiment, which...
Embodiment 2
[0070] The only difference between this example and Example 1 is that in step S2 of the method for preparing cell-penetrating peptides, the molar amount of each amino acid is 5 times the molar amount of the resin, and the molar amount of the condensing agent 10 times the molar weight of the resin.
Embodiment 3
[0072] The difference between this example and Example 1 is that in step S2 of the method for preparing cell-penetrating peptides, the molar amount of each amino acid is 1 times the molar amount of the resin, and the molar amount of the condensing agent 10 times the molar weight of the resin.
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