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Cell penetrating peptide and preparation method and application thereof

A membrane-penetrating peptide and cell technology, applied in the field of biomedicine, can solve problems such as cationic penetrating peptides that have not yet been developed, and achieve the effects of significant membrane-penetrating effect, low immunogenicity, and high membrane-penetrating efficiency.

Inactive Publication Date: 2018-11-13
肽泽(武汉)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, cationic membrane-penetrating peptides with the above functions have not yet been developed

Method used

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  • Cell penetrating peptide and preparation method and application thereof
  • Cell penetrating peptide and preparation method and application thereof
  • Cell penetrating peptide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The inventor searched and analyzed the human protein database, and found that a short peptide (RRRRRKQARRPRRRRAR) of the m6A code reader YTHDC1 protein in the human nucleus is rich in arginine and has a strong positive charge. The short peptide may be a new type of human-derived amphiphilic membrane-penetrating peptide with autonomous membrane-penetrating function, thus providing the human-derived cell-penetrating peptide (YTHDC1 for short) in this example, which includes the following sequence: RRRRRKQARRPRRRRAR , whose amino acid sequence is shown in the sequence listing; wherein, the P is selected from proline residues, the R is selected from arginine residues, the A is selected from alanine residues, and the Q is selected from Glutamine residues, said K is selected from lysine residues.

[0042] Further, the method for preparing the above-mentioned cell-penetrating peptide includes the following steps:

[0043] S1, take resin by weighing (as Fmoc-Wang resin), pour ...

Embodiment 2

[0060] The only difference between this example and Example 1 is that in step S2 of the method for preparing cell-penetrating peptides, each amino acid (including arginine, alanine, glutamine, proline and lysine ) are all 5 times the molar weight of the resin, and the molar weight of the condensing agent is 10 times the molar weight of the resin.

Embodiment 3

[0062] The only difference between this example and Example 1 is that in step S2 of the method for preparing cell-penetrating peptides, each amino acid (including arginine, alanine, glutamine, proline and lysine ) is 1 time of the molar weight of the resin, and the molar weight of the condensing agent is 10 times of the molar weight of the resin.

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Abstract

The invention discloses cell penetrating peptide and a preparation method and application thereof. The cell penetrating peptide comprises the following sequence of RRRRRKQARRPRRRRAR. The cell penetrating peptide is safe, and has obvious penetrating effect.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a human-derived cell-penetrating peptide and its preparation method and application. Background technique [0002] The body's cell membrane barrier only allows non-fat-soluble molecules with a molecular weight of less than 600Da to enter living cells. Although it has an important protective effect on the body, it also makes many promising drugs be abandoned. Cell-penetrating peptides (CPPs) are some short peptides less than 30 amino acids, which can carry molecules with molecular weight many times larger than themselves into cells. There are many ways to classify cell-penetrating peptides, which can be divided into cationic, amphiphilic and hydrophobic peptides according to their physical and chemical properties. [0003] In organisms, the positively charged sequence of the protein can enhance the binding of the protein to the RNA, thereby exerting the function of RNA regulation or al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C07K1/04C12N15/87
CPCC07K7/08C12N15/87
Inventor 唐伟郭晓霞刘岩松段茹许力柳项
Owner 肽泽(武汉)生物科技有限公司
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