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A kind of cell penetrating peptide and its preparation method and application

A technology of membrane-penetrating peptides and cell membranes, which is applied in the field of electronic biomedicine, can solve problems such as safety risks, and achieve the effects of low immunogenicity, significant membrane-penetrating effect, and high membrane-penetrating efficiency

Active Publication Date: 2020-12-08
肽泽(武汉)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Green and Frankel first observed that the TAT sequence from the HIV-1 transactivator can be efficiently absorbed by different types of cells, but because the TAT derived from the virus has clinical risks due to safety, human-derived membrane-penetrating peptides are being constantly attracting attention

Method used

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  • A kind of cell penetrating peptide and its preparation method and application
  • A kind of cell penetrating peptide and its preparation method and application
  • A kind of cell penetrating peptide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The inventors searched and analyzed the human protein database and found that a 14-amino acid short peptide (RRRTLRRRAAQRCG) of the human 3-phosphoinositide dependent protein kinase-1 variant (3-phosphoinositide dependent protein kinase-1 variant) has two parents It is speculated that this short peptide may be a new type of human-derived amphiphilic membrane-penetrating peptide with autonomous membrane-penetrating function, thus providing the human-derived cell-penetrating peptide (PDPK1 for short) in this example, which includes The following sequence: RRTLRRRAAQRCG, its amino acid sequence is shown in the sequence listing; wherein, the P is selected from proline residues, the R is selected from arginine residues, and the T is selected from threonine residues, so Said L is selected from leucine residues, said A is selected from alanine residues, said Q is selected from glutamine residues, said C is selected from cysteine ​​residues, and said G is selected from glycine r...

Embodiment 2

[0059] The difference between this example and Example 1 is that in step S2 of the method for preparing cell-penetrating peptides, the molar amount of each amino acid (including proline, glycine, arginine and lysine) is 5 times the molar weight of the resin, and the molar weight of the condensing agent is 10 times the molar weight of the resin.

Embodiment 3

[0061] The difference between this example and Example 1 is that in step S2 of the method for preparing cell-penetrating peptides, the molar amount of each amino acid (including proline, glycine, arginine and lysine) is 1 times the molar weight of the resin, and the molar weight of the condensing agent is 10 times the molar weight of the resin.

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Abstract

The invention discloses a cell penetrating peptide and a preparation method and application thereof. The cell penetrating peptide comprises the following sequence: PRTLRRRRAAQRCG. Moreover, the cell penetrating peptide is safe and obvious in cell penetrating effect.

Description

technical field [0001] The invention relates to the field of electronic biomedicine, in particular to a human-derived cell-penetrating peptide and its preparation method and application. Background technique [0002] The body's cell membrane barrier only allows non-fat-soluble molecules with a molecular weight of less than 600Da to enter living cells. Although it has an important protective effect on the body, it also makes many promising drugs be abandoned. Cell-penetrating peptides (CPPs) are some short peptides less than 30 amino acids, which can carry molecules with molecular weight many times larger than themselves into cells. There are many ways to classify cell-penetrating peptides, which can be divided into cationic, amphiphilic and hydrophobic peptides according to their physical and chemical properties. [0003] Green and Frankel first observed that the TAT sequence from the HIV-1 transactivator can be efficiently absorbed by different types of cells, but because ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C07K1/34C07K1/04C12N15/65
CPCC07K7/08C12N15/65
Inventor 郭晓霞唐伟刘岩松
Owner 肽泽(武汉)生物科技有限公司
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