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A kind of cell penetrating peptide and its preparation method and application

A technology for penetrating membrane peptides and cell membranes, applied in the field of biomedicine, can solve the problems of human pathogenicity and unsatisfactory membrane penetrating efficiency, and achieve the effects of low immunogenicity, significant membrane penetrating effect and high membrane penetrating efficiency.

Active Publication Date: 2021-01-22
肽泽(武汉)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although it has been confirmed that TAT can penetrate the cell membrane and enter the cell, its membrane penetration efficiency is not ideal, and it cannot effectively carry substances into the cell.
Moreover, because these proteins are related to viral transfection, they may be potentially pathogenic to humans and have not been used clinically.

Method used

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  • A kind of cell penetrating peptide and its preparation method and application
  • A kind of cell penetrating peptide and its preparation method and application
  • A kind of cell penetrating peptide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The inventors searched and analyzed the human protein database and found that a 12-amino acid short peptide (PGRKRRRRRKG) of human MyoD family inhibitor domain-containing protein isoform c (MyoD family inhibitor domain-containing protein isoform c) is rich in It contains arginine and has a strong positive charge. It is speculated that this short peptide may be a new type of human-derived membrane-penetrating peptide with autonomous membrane-penetrating function, and on this basis, the human-derived cell of this example is provided Membrane-penetrating peptide (MDFIC for short), which includes the following sequence: PGRKRRRRRKG, whose amino acid sequence is shown in the sequence listing; wherein, the P is selected from proline residues, and the G is selected from glycine residues; the R is selected from from arginine residues, said K is selected from lysine residues.

[0042] Further, the method for preparing the above-mentioned cell-penetrating peptide includes the fol...

Embodiment 2

[0060] The difference between this example and Example 1 is that in step S2 of the method for preparing cell-penetrating peptides, the molar amount of each amino acid (including proline, glycine, arginine and lysine) is 5 times the molar weight of the resin, and the molar weight of the condensing agent is 10 times the molar weight of the resin.

Embodiment 3

[0062] The difference between this example and Example 1 is that in step S2 of the method for preparing cell-penetrating peptides, the molar amount of each amino acid (including proline, glycine, arginine and lysine) is 1 times the molar weight of the resin, and the molar weight of the condensing agent is 10 times the molar weight of the resin.

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Abstract

The invention discloses cell membrane-penetrating peptide as well as preparation method and application thereof. The cell membrane-penetrating peptide comprises the following sequences: PGRKRRRRRRKG.Moreover, the cell membrane-penetrating peptide is safe and has an obvious membrane-penetrating effect.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a human-derived cell-penetrating peptide and its preparation method and application. Background technique [0002] The cell membrane acts as a barrier that separates the cell from its surroundings, allowing only small molecules to enter the cell. Since many drugs are hydrophilic macromolecules, biological barriers limit the clinical application of biomolecules such as nucleotides, peptides or proteins. Cell-penetrating peptides (CPPs), usually short peptides composed of multiple amino acids, can be used as transport carriers to carry substances into cells through the cell membrane barrier. CPPs can be used as a new type of non-viral carrier to transport a variety of biomolecules through biological barriers. This important discovery provides an effective carrier for biological macromolecules with therapeutic effects, and has good application prospects in research fields such as cell b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C07K1/34C07K1/04C07K1/36C07K1/14C12N15/65
CPCC07K7/08C12N15/65
Inventor 郭晓霞唐伟刘岩松
Owner 肽泽(武汉)生物科技有限公司
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