Complex microbial inoculant, and preparation method and application thereof
A compound bacterial agent and microbial technology, which is applied in the field of agricultural microorganisms, can solve the problems of unstable application effect, substandard number of effective viable bacteria, and unguaranteed product quality, so as to maintain and improve the activity of bacterial strains. Nutrient conversion rate and the effect of improving the number of beneficial microorganisms in the product and soil fertility
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Embodiment 1
[0036] A microbial composite inoculum, the raw materials of which are composed of the following components in parts by weight: 40 parts of Bacillus subtilis fermentation broth, 25 parts of Bacillus mycoides fermentation broth, 15 parts of Coral Xenocarpus fermentation broth , 15 parts of functional bacteria, 70 parts of carrier;
[0037] The specific preparation method is as follows:
[0038] Step 1. Prepare Bacillus subtilis seed liquid, Bacillus mycoides seed liquid and Coral Xenocarpa seed liquid respectively;
[0039] Step 2. Preparation of Bacillus subtilis fermentation broth
[0040] The mulberry leaf, silkworm excrement and fish meal are uniformly mixed according to the mass ratio of 10:3:1 and then ground into powder, and then passed through an 80-mesh sieve to obtain the mixed raw material powder; the mixed raw material powder, wood vinegar liquid and water according to 15:1: Mix evenly with a mass ratio of 20 to obtain Bacillus subtilis culture medium;
[0041] Inoculate the...
Embodiment 2
[0052] A microbial composite inoculum, the raw materials of which are composed of the following components in parts by weight: 50 parts of Bacillus subtilis fermentation broth, 30 parts of Bacillus mycoides fermentation broth, and 10 parts of Coral Xenocarpus fermentation broth , 10 parts of functional bacteria, 60 parts of carrier.
[0053] The preparation method is the same as in Example 1, except that the formula is changed to the formula of the microbial composite inoculum of Example 2, and the moisture content of the prepared microbial composite inoculum is 10%.
Embodiment 3
[0055] A microbial composite inoculum, the raw materials of which are composed of the following components in parts by weight: 30 parts of Bacillus subtilis fermentation broth, 20 parts of Bacillus mycoides fermentation broth, and 20 parts of Coral Xenocarpus fermentation broth , 20 parts of functional bacteria, 80 parts of carrier.
[0056] The preparation method is the same as in Example 1, except that the formula is changed to the formula of the microbial composite inoculum of Example 3, and the moisture content of the prepared microbial composite inoculum is 8%.
[0057] It should be noted that the number of effective viable bacteria in the fermentation broth of Bacillus subtilis, the number of effective viable bacteria in the fermentation broth of Bacillus mycoides, and the number of effective viable bacteria in the fermentation broth of Xenocarpus corallinea are all ≥10 11 cfu / mL;
[0058] The functional inoculum is a mixture of nitrogen-fixing bacteria and rhizobia in a mass r...
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