Breeding method for anti-G type orobanche coerulescens sunflower restoring line 1808R
A technology of sunflower and restorer line, applied in the field of plant genetics and breeding, can solve the problem that sunflower cannot be well resistant to G-type strain, and achieve the effects of ensuring high pathogenicity intensity, ensuring high resistance to strain and shortening the breeding process.
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[0034] (1) Physiological race identification of Liedang seeds: Utilize the identification host (a set of sunflower materials containing different resistance genes to Liedang) imported from abroad, adopt the method of inoculating Liedang in a culture cup, and carry out physiological tests on the collected Liedang seeds. Race identification; according to the identification of the host's anti-sensitivity response to Liedang, the corresponding table can determine the physiological race type of the Liedang sample; see Table 1 below; See the training process and judgment method Figure 1-3 ;
[0035] figure 1 It means that the culture cup is cultivated under the conditions of temperature 25-28°C, light intensity 8000-10000Lux, light duration 14h / d, and the soil humidity in the culture cup is kept at 60%-70%. When identifying the host and growing 4 leaves, the seedlings are fixed, and 1 seedling is reserved for each cup; figure 2 It means that 45 days after the emergence of the i...
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