Arsenic chelating type immune compound with excellent effect and preparation method thereof

Abstract

The invention discloses an arsenic chelating type immune compound. The arsenic chelating type immune compound is selected from one of the following compounds: the compound formed by binding arsenic ions and the immune compound; or, the compound formed from the arsenic ions bound with vector protein and an antibody specifically bound with the vector protein; or, the compound formed by binding the arsenic ions with immune globulin and then binding with the vector protein. The invention also discloses a preparation method of the arsenic chelating type immune compound with excellent effect. The preparation method comprises the following steps of S1, preparing a chelating agent solution; S2, preparing a vector protein solution; S3, stirring and staying overnight; S4, dialyzing; S5, adding the arsenic ions; S6, recycling the waste solution; S7, specifically binding. The preparation method has the characteristics that the application range is broader, the cost is reduced, the dialyzing rate is increased, the preparation cycle is shortened, the energy-saving and environment-friendly effects are realized, and the chemical pollution is avoided; the market competitiveness is larger.

Description

technical field

[0001] The invention relates to the field of chelates, in particular to an arsenic chelating immune complex with excellent effect and a preparation method thereof. Background technique

[0002] Chelates are widely used in mineral flotation process, hydrometallurgy, extraction and separation of metal elements, catalytic synthesis of substances, softening of water, electroplating process, pharmaceutical industry, dyeing process, etc.; the existing arsenic chelate The preparation method of the compatible immune complex is not perfect, and the scope of application is not wide enough. It is inconvenient to complete the preparation process of the target in a small laboratory, and the number of dialysis will prolong the preparation period of the target to a certain extent. There is no clear treatment of ions and other wastes in the waste liquid, and the discharge of ions and other wastes in the waste liquid will cause chemical pollution if they are not treated up to...

Examples

preparation example Construction

[0033] A method for preparing an arsenic chelating immune complex as claimed in claim 1, comprising the following steps:

[0034] S1: First prepare the chelating agent solution, and then dissolve the chelating agent in the solvent to obtain the chelating agent solution;

[0035] S2: Take an appropriate amount of carrier protein, and add the carrier protein into the borate buffer solution to prepare the carrier protein solution;

[0036] S3: Add the prepared chelating agent solution into the carrier protein solution, place in a constant temperature water bath and stir evenly, and keep the constant temperature water bath for 12 hours to obtain the mixed solution;

[0037] S4: Take the dialysis bag and add EDTA-NaHCO3 solution into the dialysis bag to boil, fix the dialysis bag in the middle of the water tank, and add enough ddH2O to the water tank, the volume ratio of the ddH2O to the solution in the dialysis bag is 98:1, repeat this step twice, put the mixture in S3 into a dia...

technical field

[0001] The invention relates to the field of chelates, in particular to an arsenic chelating immune complex with excellent effect and a preparation method thereof. Background technique

[0002] Chelates are widely used in mineral flotation process, hydrometallurgy, extraction and separation of metal elements, catalytic synthesis of substances, softening of water, electroplating process, pharmaceutical industry, dyeing process, etc.; the existing arsenic chelate The preparation method of the compatible immune complex is not perfect, and the scope of application is not wide enough. It is inconvenient to complete the preparation process of the target in a small laboratory, and the number of dialysis will prolong the preparation period of the target to a certain extent. There is no clear treatment of ions and other wastes in the waste liquid, and the discharge of ions and other wastes in the waste liquid will cause chemical pollution if they are not treated up to...

Examples

preparation example Construction

[0033] A method for preparing an arsenic chelating immune complex as claimed in claim 1, comprising the following steps:

[0034] S1: First prepare the chelating agent solution, and then dissolve the chelating agent in the solvent to obtain the chelating agent solution;

[0035] S2: Take an appropriate amount of carrier protein, and add the carrier protein into the borate buffer solution to prepare the carrier protein solution;

[0036] S3: Add the prepared chelating agent solution into the carrier protein solution, place in a constant temperature water bath and stir evenly, and keep the constant temperature water bath for 12 hours to obtain the mixed solution;

[0037] S4: Take the dialysis bag and add EDTA-NaHCO3 solution into the dialysis bag to boil, fix the dialysis bag in the middle of the water tank, and add enough ddH2O to the water tank, the volume ratio of the ddH2O to the solution in the dialysis bag is 98:1, repeat this step twice, put the mixture in S3 into a dia...

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