A new type of counter construction method
A construction method and technology of counters, applied in the field of counters, can solve the problems of difficulty in realizing counter functions and complex sequential logic circuit methods, and achieve the effects of simple and easy logic operation, large switching ratio, and easy distinction.
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Embodiment 1
[0032] 1. The positive counting construction process of the counter
[0033] Take 4 EP tubes, numbered 1-4 and do the following:
[0034] At a concentration of 1 x 10 -3 mol / L, Tris-HCl buffer solution with pH=6.0 is the initial state of positive counting, and the concentration of 100×10 -6 mol / L cyanine dye Ⅰ solution and the concentration is 1×10 -3 mol / L, Tris-HCl buffer solution of pH=6, make the mixed solution volume be 1mL, obtain solution 1; Wherein, the concentration of cyanine dye I solution in solution 1 is 1×10 - 6 mol / L.
[0035] 2-4 tubes contain a concentration of 1 × 10 -3 mol / L, Tris-HCl buffer solution with pH=6 and a concentration of 100×10 -6 mol / L solution of cyanine dye Ⅰ, using equal parts of potassium ions as forward pulse signal, add one equal part of potassium ions to No. 2 tube, add two equal parts of potassium ions to No. 3 tube, and add Add three equal parts of potassium ions to make the volume of the mixed solution 1mL to obtain solution 2-4...
Embodiment 2
[0063] 1. The positive counting construction process of the counter
[0064] Take 4 EP tubes, numbered 1-4 and do the following:
[0065] At a concentration of 100 x 10 -3 mol / L, Tris-HCl buffer solution with pH=9.0 is the initial state of positive counting, and the concentration of 1×10 -3 mol / L cyanine dye II solution and concentration of 100×10 -3 mol / L, Tris-HCl buffer solution of pH=9, make the mixed solution volume be 1mL, obtain solution 1; Wherein, the concentration of cyanine dye II solution in solution 1 is 20×10 -6 mol / L.
[0066] 2-4 tubes contain a concentration of 100×10 -3 mol / L, Tris-HCl buffer solution with pH=9 and a concentration of 1×10 -3 mol / L cyanine dye II solution, using equal parts of potassium ions as the forward pulse signal, add one equal part of potassium ions to No. 2 tube, add two equal parts of potassium ions to No. 3 tube, and add Add three equal parts of potassium ions to make the volume of the mixed solution 1mL to obtain solution 2-4;...
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