Crack-type quantitative easily-marked hand-held surface sampling culture dish adopting grid counting method

Abstract

The invention discloses a crack-type quantitative easily-marked hand-held surface sampling culture dish adopting a grid counting method. The culture dish comprises a dish bottom and a dish cover; a transverse crack and a longitudinal crack formed in a first dish bottom side wall can be cracked when the stress is exerted, so that a movable dish bottom side wall is broken away from the culture dish,and the height of the first dish bottom side wall is lower than the plane of a solid medium, and therefore, an operation of surface microorganisms sampling is performed more simply and more conveniently. Additionally, a mark zone enables the marking work to be more efficient, minute bacterial colonies can be observed more conveniently through an observation window, scale marks can be used for indicating the pouring volume of an agar medium, and a counting process of bacterial colonies can be simplified by grid lines.

Description

technical field [0001] The invention relates to an experimental equipment, in particular to a split-type quantitative and easy-to-mark visual grid counting surface sampling culture dish. Background technique [0002] Petri dish is one of the most widely used experimental equipment in biology. It is often used for the culture test of microorganisms on the surface of food packaging materials, tableware, etc., and the sampling of microorganisms on the surface of the object is usually wiped with a sterile cotton swab on the surface of the object to be tested, or Use sterile filter paper to stick to the surface of the object to be tested, and then transfer the cotton swab or filter paper to the buffer solution for eluting to achieve the purpose of sampling. However, cotton swabs or filter paper cannot completely release the collected microorganisms, which may easily cause incomplete cultivation of cultivable microorganisms; in addition, the sampling of microorganisms on the surfa...

AI Technical Summary

Problems solved by technology

However, cotton swabs or filter paper cannot completely release the collected microorganisms, which may easily cause incomplete cultivation of cultivable microorganisms; in addition, the sampling of microorganisms on the surface of objects requires aseptic operation, while cotton swabs or filter paper are used in the collection and elution of microorganisms. During the process, it is easy to be polluted by environmental microorganisms, and it is difficult to meet the requirements of aseptic operation; these shortcomings will cause the deviation of the microbial test results on the surface of the object

In addition, the operation process of the above method is cumbersome and inefficient, which easily causes waste of human resources

Most of the existing petri dishes are made of glass and need to be marked with an oil-based marker pen. However, the handwriting of the oil-based marker pen needs to be erased with a special organic solvent. Therefore, when multiple petri dishes are used repeatedly, the inconvenience of marking will easily cause to confuse

When the culture dish is poured into the agar medium, the pouring volume of the medium is usually determined by the operator according to experience, so the pouring volume of the agar medium of different personnel may have a large difference, thereby affecting the relevant experimental results

When the colony of the target culture grown on the medium in the petri dish is small, it is necessary to enlarge the colony with a microscope to observe the accurate shape of the colony, and the petri dish needs to be opened during the magnification and observation of the colony, which may cause pollution or spillage of harmful cultures

When the number of colonies of the target culture grown in the culture medium in the petri dish is huge, the counting process of the colonies is more cumbersome

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