A fermentation process of Bacillus licheniformis and its use in the preparation of microbial agents and bio-organic fertilizers for preventing cucumber gray mold

Abstract

The invention discloses a fermentation process of Bacillus licheniformis and the preparation of microbial agent and bio-organic fertilizer for preventing gray mold of cucumber. Against the background of broken eggshells and shredded newspaper scraps, manganese sulfate can be slowly released in the medium, and the distribution is uniform, with remarkable effects. Cooperating with related processes can significantly increase the speed of transsporation, shorten the cultivation time, and save costs; the melon pedicle extract is toxic, Cooperating with relevant processes can not only eliminate toxicity, ensure no miscellaneous bacteria, but also release nutrients such as glucose, galactose, and amino acids contained in melon stalks. The prepared bio-organic fertilizer and the Bacillus licheniformis contained in microbial agents are bio-control Bacteria, which can effectively prevent gray mold of cucumber. Bio-organic fertilizer can effectively promote the development of root system, make cucumber plants strong, and enhance the ability to resist gray mold; spraying microbial agents on the leaves can form a protective layer on the leaves to prevent the infection of Botrytis cinerea, and play a role in cucumber leaves. Protective effects.

Description

technical field [0001] The invention relates to a Bacillus licheniformis fermentation process and a method for preventing gray mold of cucumber. The preservation number of the strain is CGMCC1.813. The strain is a biocontrol bacterium, which has obvious inhibitory effect on Botrytis cinerea. The invention uses a bacillus licheniformis as a functional bacterium to prepare a bio-organic fertilizer and a microbial agent, which has a preventive effect on cucumber gray mold. Background technique [0002] Botrytis cinerea is a disease that occurs perennially in protected cultivation of cucumbers, and it has become more serious in recent years. Because the fruit is often infested and causes rot, it is often called fruit rot disease and mildew rot disease. The disease is caused by the infection of Botrytis cinerea Pers.ex Fr. Overwinter or oversummer with sclerotia in soil or on diseased residues with mycelium and conidia. The conditions in the following spring are suitable, the...

AI Technical Summary

Problems solved by technology

The traditional method of treating this disease is to spray the infected parts with pesticides, resulting in pesticide residues, environmental pollution, and endangering human health.

And the traditional use of large chemical fertilizers makes the soil increasingly poor, resulting in underdeveloped cucumber roots, weak growth and weak disease resistance

The focus of Botrytis cinerea is on prevention. Infection will inevitably lead to a decline in the yield and quality of cucumbers, affecting the income of vegetable farmers

Images