Reagent used for diagnosing children cytomegalovirus infection

Abstract

The invention discloses a reagent used for diagnosing children cytomegalovirus infection. The reagent comprises the urine of a patient, wherein the urine is centrifuged for 8-10 min at 2500 r / min to remove cell fragments, supernate is collected, polyethylene glycol is used for preprocessing since the urine contains PCR (Polymerase Chain Reaction) inhibitor, 50 mu l of urine supernatant and 50 mu lof 20% polyethylene glycol and 25 mu l of 2mol / L of NaCl are mixed, and mixture is put in an ice-bath for 5-6 h; centrifugation is carried out for 25-30 min at 15000 r / min to collect precipitates; centrifugation is carried out for 2-3 min at 6400 r / min; the supernatant is sucked as much as possible, and the precipitates are suspended in distilled water; suspension can be directly used for PCR amplification; during amplification, heating is carried out at 100 DEG C for 8-10 min, and the obtained product is quickly cooled in the ice bath. By use of the reagent, the urine of the patient is collected as a sample, viruses are separated from clinic samples, antibodies are increased in four times or more, or continuous antibody titer is raised so as to be favorable for improving a diagnosis effect.

Description

technical field [0001] The invention relates to the technical field of cytomegalovirus, in particular to a reagent for diagnosing childhood cytomegalovirus infection. Background technique [0002] Human cytomegalovirus (HCMV) belongs to the herpesviridae family and is the largest virus in the human herpesvirus group, which can cause human cytomegalovirus infection. Cytomegalovirus is generally susceptible in the population, especially some children and newborns, usually without obvious symptoms, latent in the host cells, which is a recessive infection, and when the immune function is low or defective, it often presents a challenge infection (dominant infection) Infection), the virus can invade multiple organs and systems, causing severe disease and death in severe cases. Therefore, early monitoring of HCMV latent and active infection status is very important for immunocompromised patients. [0003] However, due to the ubiquity of HCMV infection in the human body, it usuall...

AI Technical Summary

Problems solved by technology

However, due to the particularity of the methodology, the completion of the whole process is affected by many factors, including: specimen processing time, choice of fixative, specificity of monoclonal antibody cell lines, etc. In addition, this method requires high technical requirements for microscopic examination personnel. , the method is difficult to achieve high-throughput detection, the anti-pollution ability is not strong, the cost is too high, and it is difficult to popularize at present

[0007] In summary, there is currently no convenient and clear method to clearly distinguish between latent and provocative infection of HCMV.