Biological sensing detection method used for Huso huso fry identification

Abstract

The invention discloses a biological sensing detection method used for Huso huso fry identification. According to the biological sensing detection method used for Huso huso fry identification, DNA offry to be measured is taken as a template for probe extension so as to obtain a probe extension product; biological sensing method is adopted to detect that whether the probe extension product can beanchored onto the surface of an electrode modified with an anchored receptor using an anchoring labelled molecule; if the detected results are positive, the dry to be measured is Huso huso fry. The biological sensing detection method comprises following steps: in probe extension, dUTP provided with the anchoring labelled molecule is connected with a probe with a nucleotide sequence represented bySEQ ID NO:3 so as to obtain the probe extension product of the Huso huso fry in the fries to be detected. The biological sensing detection method is capable of identifying specie source in sturgeon fry introduction, and assisting culture organizations to perform cultured objects and processed products grading.

Description

technical field [0001] The invention relates to the technical field of aquatic organisms, in particular to a biosensing detection method for the identification of beluga seedlings. Background technique [0002] Sturgeon is the collective name for fish of the order Acipenseriformes. There are 27 species in 2 families, 6 genera, and 27 species in the world, all of which have been included in the list of protected species under the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). Fish and its products mainly rely on artificial breeding. In this context, my country's sturgeon farming industry is booming, with annual output increasing from 9,400 tons in 2003 to 89,800 tons in 2016, ranking first in the world's largest sturgeon farming country, accounting for more than 80% of the world's total output . Since 2017, sturgeon has been included in the national modern agricultural industrial technology system (special freshwater fish system). ...

AI Technical Summary

Problems solved by technology

[0005] Congiu et al. used AFLP molecular markers to identify sturgeon and caviar products. Chelomina et al. used RAPD molecular markers to identify hybrids in natural populations of Acipenser schrenckii. Dong Chuanju et al. developed PCR based on mitochondrial DNA. -RFLP molecular identification method, but the existing methods require electrophoresis to detect the product. Improper operation will pose health risks to the inspectors, and experimental waste that has not been safely treated may also cause environmental pollution. Therefore, it is only suitable for professional experiments room, not suitable for on-site testing

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