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A protein-wrapped fluorescent gold nanocluster, its preparation method and its application

A technology of fluorescent gold nanometers and gold nanoclusters, which is applied in the field of preparation of protein-wrapped fluorescent gold nanoclusters, can solve problems such as complex reaction conditions, and achieve broad application prospects, good biocompatibility, detection function and therapeutic potential. Effect

Active Publication Date: 2021-04-13
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The commonly used preparation method of gold nanocluster is to use thiol compound as protective agent and sodium borohydride as reducing agent to prepare by reducing the precursor of gold. The reaction conditions of the above preparation method are complex

Method used

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  • A protein-wrapped fluorescent gold nanocluster, its preparation method and its application
  • A protein-wrapped fluorescent gold nanocluster, its preparation method and its application
  • A protein-wrapped fluorescent gold nanocluster, its preparation method and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Take 0.1 mL of 25 mM HAuCl 4 Add it into 2.36mL of 0.53mM aprotinin solution, and mix it thoroughly with a vortexer for 5min; then add 0.08mL of 1.0M NaOH solution into the tube, and mix it quickly with a vortexer to avoid precipitation. The mixing time is 5min. into a synthetic solution; the synthetic solution was airtight and protected from light, and shaken on a shaker at 90 rpm at 37° C. for 12 hours. After the reaction was completed, the sample was dialyzed in a 1000Da dialysis bag for 10 h, and then stored in the dark at 4°C for later use. Aprotinin and HAuCl 4 The molar ratio is 1:2.

[0035] The Ap-Au NCs that embodiment 1 makes, its morphology is as follows figure 1 As shown, it is a spherical shape with uniform size;

[0036] Such as figure 2 Shown: 118 Ap-Au NCs of gold nanocluster particles were measured, and the particle size distribution of Ap-Au NCs was found to be in the range of 2.25nm-3.25nm, and the average particle size was 2.84±0.6nm.

[0037]...

Embodiment 2

[0040] Take 0.1 mL of 25 mM HAuCl 4 Add it to 1.57mL of 0.53mM aprotinin solution, and mix it thoroughly with a vortex for 8 minutes; then add 0.06mL of 1.0M NaOH solution into the tube, and mix it quickly with a vortex to avoid precipitation, and mix for 8 minutes; The synthesized solution was shaken on a shaker at 90 rpm at 37° C. for 12 hours under airtight conditions and protected from light. After the reaction, the sample was dialyzed in a 1000Da dialysis bag for 36 hours, and then stored at 4°C in the dark until use. Aprotinin and HAuCl 4 The molar ratio is 1:3.

Embodiment 3

[0042] Take 0.1 mL of 25 mM HAuCl 4 Add it to 3.14mL of 0.53mM aprotinin solution, and mix it thoroughly with a vortex for 10 minutes; then add 0.1mL of 1.0M NaOH solution to the tube, and mix it quickly with a vortex to avoid precipitation, and mix for 10 minutes; The synthesized solution was shaken on a shaker at 90 rpm at 37° C. for 24 hours under airtight conditions and protected from light. After the reaction was completed, the sample was dialyzed in a 1000Da dialysis bag for 16 hours, and then stored in the dark at 4°C for later use. Aprotinin and HAuCl 4 The molar ratio is 1:1.5.

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Abstract

The invention discloses a protein-wrapped fluorescent gold nanocluster, a preparation method and an application thereof. The gold nanocluster is a gold nanocluster wrapped by aprotinin, and the gold nanocluster is composed of aprotinin molecule and Au22 or Au21 or Au16 composition. Compared with the prior art, the present invention not only has fluorescence, but also the activity of aprotinin itself is not affected, and can be applied to the fields of metal ion detection, biological imaging and protease detection.

Description

technical field [0001] The invention belongs to gold nanoclusters, preparation methods and applications thereof, in particular to the technical field of protein-wrapped fluorescent gold nanoclusters, preparation methods and applications thereof. Background technique [0002] Gold nanoclusters are generally composed of dozens to hundreds of gold atoms, and the size is within 2nm. The physical and chemical properties of gold nanoclusters depend on the particle size of the nanoclusters. As the atomic number and size of gold nanoclusters change, the fluorescence emission wavelength varies from ultraviolet (UV) to near-infrared (IR) region. Gold nanoclusters have unique advantages such as good stability, photofluorescence, photostability, strong fluorescence emission rate, and good biocompatibility. They have become one of the most promising materials in the field of nanomaterials and are widely used in Detection of metal ions and small molecules, biomarkers, cell imaging, enzym...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64B22F9/24B22F1/02B82Y30/00B82Y5/00C09K11/02C09K11/58B82Y40/00
CPCG01N21/643G01N21/6486C09K11/025C09K11/58B82Y5/00B82Y30/00B82Y40/00B22F9/24G01N2021/6432B22F1/07B22F1/102
Inventor 王宝娟高鹏李全发鲁山刘冯楠张婷吴爽闫浩马晓燕王鹏
Owner ANHUI NORMAL UNIV
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