Method for establishing zebrafish senile dementia model and application

A technology of zebrafish and disease, which is applied in the field of animal model preparation, can solve the problems of long breeding cycle, high cost of rat culture, difficulty in realizing rapid activity of compounds, high-throughput screening, etc., so as to reduce the amount of drugs and save experiments resource effect

Active Publication Date: 2018-12-07
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In one embodiment, four weeks of infusion of a solution of Fe2+, Aβ42, buthionine sulfoximine (FAB) into the left ventricle of young adult Long-Evans rats induced memory impairment accompanied by elevated levels of hyperphosphorylated Tau protein in the cerebrospinal fluid. High, the rats used in this patent have a high cost of culture and a long breeding cycle, and it is difficult to achieve rapid and high-throughput screening of compound activity

Method used

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  • Method for establishing zebrafish senile dementia model and application
  • Method for establishing zebrafish senile dementia model and application
  • Method for establishing zebrafish senile dementia model and application

Examples

Experimental program
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Effect test

Embodiment 1

[0043] The method for establishing the zebrafish senile disease model, the steps are as follows:

[0044] (1) Add the water culture solution and the glucose culture solution containing 2% mass percentage concentration into different 3L culture tanks respectively, and the volume of the culture solution in each tank is 1.5L. Select healthy 7-month-old wild-type zebrafish and put them in water culture. Put 10 zebrafish in each tank, and culture them under light control for 24 hours at 23°C (14 hours of light, 10 hours of darkness), take out the zebrafish and wash them with water culture solution for 5 times, then put them into the glucose culture solution, and continue to Light-controlled culture was carried out at 23°C for 24 hours (14 hours of light and 10 hours of darkness), and then the zebrafish was taken out and washed 5 times with water culture solution, then put into the water culture solution, and continued to be alternately cultured in the two culture solutions for 60 da...

Embodiment 2

[0048] The method for establishing the zebrafish senile disease model, the steps are as follows:

[0049] (1) Add the water culture solution and the glucose culture solution containing 1% mass percentage concentration into different 3L culture tanks respectively, and the volume of each tank culture solution is 1.5L, and select healthy 7-month-old wild-type zebrafish and put them in water culture Put 10 zebrafish in each tank, and culture them under light control at 23°C for 24 hours (light for 12 hours, dark for 12 hours), take out the zebrafish and wash them with water culture solution for 3 times, then put them into the glucose culture solution, and continue to Light-controlled culture was carried out at 23°C for 24 hours (12 hours of light and 12 hours of darkness), then the zebrafish were taken out and rinsed three times with water culture solution, then put into the water culture solution, and continued to alternately culture in the two culture solutions for 60 days. Feed...

experiment example 1

[0067] The zebrafish after the treatment of Example 1, Example 2, Comparative Example 1, Comparative Example 2, and Comparative Example 3 were respectively put into a 2L culture tank, and 3 / 4 volume of water culture solution was added in the tank, and one piece was placed at a time. On the detection platform of the zebrafish behavioral analysis system, turn on the background light source, and use the Zeblab software to collect the movement track of the zebrafish within 30 minutes in the vertical direction, such as figure 1 Shown, and utilize software to carry out segmental measurement to parameters such as the average total swimming distance of each zebrafish in embodiment 1, embodiment 2, comparative example 1, comparative example 2, comparative example 3, sluggish frequency and upper layer residence time , taking every 5min as a time period (T 0-5 ), choose T 15-20 data, such as figure 2 As shown, the state of zebrafish in this time period is relatively stable. According ...

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Abstract

The invention particularly relates to a method for establishing a zebrafish senile dementia model and application. A glucose culture solution and an okada acid culture solution are included,the glucose culture solution and the okada acid culture solution are alternately used for culturing zebrafishes, and a single culture time of the glucose culture solution orthe okada acid culture solution is 22-26 hours. The method disclosedis the first methodwhich alternately cultures the zebrafishes with a specific concentration of the glucose culture solution and the okada acid culture solution, zebrafish senile dementia-like symptoms can be quickly and effectively caused, thereby greatly reducing the dosage of okada acid, and experimental resources are saved.

Description

technical field [0001] The invention specifically relates to a method for establishing a zebrafish senile disease model and its application, and belongs to the technical field of animal model preparation. Background technique [0002] Alzheimer's disease (AD) is an aging central nervous system degenerative disease characterized by mental decline. With the aggravation of population aging, its incidence rate increases year by year. Usually after the age of 65, the onset is slow, manifested as cognitive dysfunction, mainly memory impairment, which is progressively aggravated and difficult to reverse, eventually leading to dementia and dysfunction, which seriously affects the cognitive function and living ability of the elderly, and aggravates family members and family members. Social care and financial burden. Therefore, early diagnosis, timely treatment and effective prevention have become important clinical and scientific research issues. There is currently no effective dr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A23K50/80A23K20/163A23K20/121A23K20/22A23K20/24
CPCA01K61/10A01K2227/40A01K2267/0312A23K20/121A23K20/163A23K20/22A23K20/24A23K50/80Y02A40/81
Inventor 王雪刘可春何秋霞韩利文张云盛文龙李晓彬孙晨
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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