The invention relates to a low-temperature platform for in vitro electrophysiological recording. The low-temperature platform includes a perfusion slot, a silicone hose, an aluminum tube, a peristaltic pump, a temperature control box, a magnetic table base, a low-temperature fixed platform, and a refrigeration aluminum plate; The perfusion card slot is located on the refrigeration aluminum plate; the refrigeration aluminum plate is fixed on the low-temperature fixed platform through a fixing clip, and the low-temperature solid platform is provided with an ice tank body, a fixed adjustment knob, and a magnetic table seat; the perfusion There is a perfusate outlet on the card slot, and a silicone hose is connected to the outlet of the perfusate fluid, and the other end of the silicone hose is placed in the liquid carrier; two aluminum tube interfaces are provided on the side of the perfusion card slot, and one of the aluminum tubes is The interface is connected to the peristaltic pump through the aluminum tube, and the peristaltic pump is connected to the refrigeration aluminum ring through the aluminum tube, and the other aluminum tube interface is connected to the refrigeration aluminum ring through the aluminum tube; its advantages are: temperature control, high heat dissipation efficiency, Strong anti-interference ability, convenient installation, simple operation, controllable perfusion speed, and low cost.