Pharmaceutical composition for controlling ovulation time period of donkey to be relatively centralized and application method thereof

Abstract

The invention discloses a pharmaceutical composition for controlling the ovulation time period of a donkey to be relatively centralized and an application method thereof. According to the pharmaceutical composition, 1 part of pharmaceutical composition is powder formed by mixing 600 international units of human chorionic gonadotropin and 10 international units of follicle-stimulating hormones. According to the pharmaceutical composition and the application method thereof, in the using process, the follicular development diameter of the donkey is monitored in real time, and when the folliculardevelopment diameter of the donkey is found to reach 35 mm, a subcutaneous dosage of 50 parts of mixed powder is administrated to the donkey at a time, wherein the 50 parts of mixed powder are required to be diluted by using normal saline; and at the 32nd hour and the 42nd hour after the powder is administrated, the donkey is inseminated once separately so as to achieve the purpose of increasing the conception rate in estrus. The pharmaceutical composition has the advantages that the ovulation time of the donkey can be controlled, the abortion rate of the donkey can be decreased, term deliveryof the female donkey can be ensured, the physical development of donkey foals after delivery is good, and the survival rate is higher.

Description

technical field [0001] The invention belongs to the field of equine animal reproduction, in particular to a pharmaceutical composition for controlling relatively concentrated ovulation time periods of donkeys. Background technique [0002] Due to the unique structure of the ovaries of horses, the duration of estrus changes greatly after the appearance of estrus, which generally lasts for 3 to 14 days. There is a big difference, the ovulation time is mostly on the 3rd to 10th day of estrus, and the ovulation varies from 12 to 24 hours before the cessation of estrus, which makes it difficult to determine the time of donkey insemination. [0003] It has been reported that gonadotropin-releasing hormone or chorionic gonadotropin was used to treat donkeys or horses to promote ovulation and improve conception rate. For example, a donkey embryo transfer method disclosed in Chinese Patent Application No. 2016108107595; another example is a fresh sperm artificial insemination method...

AI Technical Summary

Problems solved by technology

However, in practice, it is found that the ovulation time of donkeys is difficult to meet the purpose of increasing the conception rate regardless of gonadotropin-releasing hormone or chorionic gonadotropin treatment alone.

[0004] In order to increase the conception rate, Chinese Patent Application No. 2014100851723 discloses a pharmaceutical composition for inducing superovulation in horses, which uses simple chorionic gonadotropin combined with follicle-stimulating hormone as an exogenous hormone. It can be seen from this document that the exogenous hormone The amount of chorionic gonadotropin and follicle-stimulating hormone in the medium is both 1000-2500 units. Although this combination achieves the purpose of superovulation in equine animals, the increase in the number of ovulations will cause twins, and donkeys often have twins. Miscarriage is easy, even if it can give birth at full term, the proportion of born donkeys with poor physical development is very high, resulting in early death, so satisfactory results cannot be achieved

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