Detection method and detection kit for HLA-B*5801 gene

Abstract

The invention relates to a detection method and detection kit for an HLA-B*5801 gene. The detection method comprises the following steps: (1) designing specific primers aiming at the HLA-B*5801 gene;(2) amplifying a target segment containing the HLA-B*5801 gene by utilizing specific PCR (Polymerase Chain Reaction); (3) digesting a PCR product segment by utilizing restriction endonuclease; (4) carrying out single base extension reaction; (5) carrying out desalting and purification treatment; and (6) analyzing gene sequences of the target gene HLA-B*5801 through a nucleic acid mass spectrometer. The detection method for the HLA-B*5801 gene has the advantages of high detection accuracy, high experiment repeatability, great flux and low cost. The invention further provides a detection kit forthe HLA-B*5801 gene; and the detection kit comprises a specific primer pair for amplifying the HLA-B*5801 gene. According to the detection kit for the HLA-B*5801 gene, an experiment flow is simplified, the operation time of workers is short, the difficulty is small and the automation degree of experiments is high.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a detection method and a detection kit for the HLA-B*5801 gene. Background technique [0002] With the sharp increase in the intake of high-protein, high-purine, and high-calorie foods in people's diets, as well as increased alcohol consumption and reduced activity levels, obesity and metabolic syndrome patients have increased, resulting in accompanying hyperuricemia and the incidence of primary gout are on the rise. Studies have shown that gout is caused by abnormal metabolism of purine substances, excessive uric acid production or poor excretion of uric acid, resulting in elevated uric acid in the blood, and deposition of urate crystals in the synovium, synovial bursa, cartilage and other tissues. Recurrent inflammatory disease, which is characterized by limited joint movement, heat, pain, and swelling, accompanied by joint deformities, nodular gout stones, and ev...

AI Technical Summary

Problems solved by technology

At present, the methods for detecting HLA-B*5801 alleles at home and abroad mainly include DNA direct sequencing and Taqman probe method. DNA direct sequencing is the current gold standard for allele detection, but it is time-consuming and laborious. , is a low-throughput detection method that is not suitable for a large number of samples; Taqman is a highly specific quantitative PCR technology, which is characterized by being suitable for the detection of a small number of alleles in large samples, and is a medium-throughput method. Allele detection, but for the simultaneous detection of multiple allele sites, the cost is high and it is not suitable for use

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