Detection kit for continuously monitoring lactic dehydrogenase isozyme I

A technology for detecting kits and lactate dehydrogenase, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that the sixth zone cannot be displayed and the detection cost is high, and achieve high cost performance, clear separation and accuracy high effect

Inactive Publication Date: 2018-12-11
浙江强盛生物科技有限公司
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  • Abstract
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Problems solved by technology

Their disadvantages are: 1. five electrophoresis bands can be separated from the serum of normal people or general patients, and the sixth zone should appear in patients with liver disease on the verge of death, but the agarose gel plate of the above kit is in During electrophoresis, the lactate dehydrogenase isoenzyme in serum can only be separated into five bands, and the sixth band cannot be displayed; 2. The detection cost is high

Method used

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  • Detection kit for continuously monitoring lactic dehydrogenase isozyme I
  • Detection kit for continuously monitoring lactic dehydrogenase isozyme I
  • Detection kit for continuously monitoring lactic dehydrogenase isozyme I

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Embodiment Construction

[0014] The preferred embodiments of the present invention are described in detail below, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, so as to define the protection scope of the present invention more clearly.

[0015] A continuous monitoring lactate dehydrogenase isoenzyme I detection kit, including R1 reagent and R2 reagent;

[0016] The R1 reagent consists of:

[0017]

[0018] The R2 reagent consists of:

[0019]

[0020] The biological buffer 1 is a TES buffer; the Chinese full name of the TES is N-3-(hydroxymethyl)methyl-2-aminoethanesulfonic acid.

[0021] The preservative is sorbic acid.

[0022] The biological buffer 2 is a HEPPSO buffer; the Chinese full name of the HEPPSO is 4-(2-hydroxyethyl)piperazine-1-2-hydroxypropanesulfonic acid.

[0023] Linear range: 2-150U / L, r≥0.990; Linear deviation: within the range of 2U / L~50U / L, the maximum absolute deviation of linearity should be ≤5U / ...

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Abstract

The invention discloses a detection kit for continuously monitoring lactic dehydrogenase isozyme I. The detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from L-guanidine thiocyanate lactate biological buffer solution 1, preservatives and albumin bovine serum; the reagent R2 is prepared from oxidized coenzyme I potassium ferrocyanide biological buffer solution 2, and albumin bovine serum. The detection kit has the effects of high speed, sensitivity, high accuracy, high specificity and deterioration and volatilization difficulty.

Description

technical field [0001] The invention relates to a continuous monitoring kit for detecting lactate dehydrogenase isoenzyme I. Background technique [0002] Clinically, in the diagnosis and treatment of certain diseases such as the cardiovascular system, respiratory system, and digestive system, it is often necessary to monitor the lactate dehydrogenase isoenzyme, and reflect the possible related diseases according to the activity changes of enzymes in different zones . Most of the lactate dehydrogenase isoenzyme monitoring kits currently used are imported from abroad, and the kits include agarose gel plates, substrates, chromogenic reagents, and chromogenic solutions. The agarose gel plate electrophoresis separates lactate dehydrogenase isozymes in serum into different electrophoresis zones; the substrate and the chromogenic agent are powders, which are dissolved in the chromogenic solution and then assisted on the gel plate, and the enzyme and the substrate occur The react...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573
CPCG01N33/573
Inventor 钱静朱天明徐俊侠
Owner 浙江强盛生物科技有限公司
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