Application of LncRNA RET in regulating and controlling radiosensitivity of tumor cells

A tumor cell, tumor cell apoptosis technology, applied in the application field of LncRNARET regulating tumor cell radiosensitivity, can solve the problem of unclear role and functional mechanism

Active Publication Date: 2018-12-18
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the biological functions of some lncRNAs have been reported, many roles and functional mechanisms are still unclear.

Method used

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  • Application of LncRNA RET in regulating and controlling radiosensitivity of tumor cells
  • Application of LncRNA RET in regulating and controlling radiosensitivity of tumor cells
  • Application of LncRNA RET in regulating and controlling radiosensitivity of tumor cells

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Embodiment 1

[0065] Example 1. Research on the role of LncRNA RET in radiosensitivity of tumor cells

[0066] LncRNA RET is a new radiation-induced high-expression LncRNA screened by the inventors earlier with gene chips, and it is named LncRNA RET (LncRNA radiation-elevated transcript). The full-length sequence of LncRNA RET (http: / / www.ncbi.nlm.nih.gov / nuccore / NR_033874.1) is shown in SEQ ID No.1.

[0067] 1. The effect of ionizing radiation on the expression of LncRNA RET

[0068] 1. Dose-effect relationship of LncRNA RET expression in tumor cells after radiation exposure

[0069] The present invention first detects the dose-effect relationship expressed by LncRNA RET after being irradiated, and it is used for HeLa cells and A549 cells 60 Coγ-ray irradiation, the set dose is 0Gy, 2Gy, 4Gy, 6Gy, 8Gy. 48h after irradiation, RNA was extracted, and the expression of LncRNA RET was detected by RT-qPCR. details as follows:

[0070] (1) Prepare the cells required for the experiment, inocu...

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Abstract

The invention discloses an application of LncRNA RET in regulating and controlling the radiosensitivity of tumor cells. The invention provides an application of RET-related biomaterials in preparationof products for regulating and controlling the radiosensitivity of the tumor cells; the RET-related biomaterials are RET or substances capable of promoting / inhibiting RET expression. With downregulation of RET expression, the growth of the tumor cells under radiation pressure can be inhibited and the radiosensitivity of the tumor cells can be increased; a possible mechanism comprises promotion oftumor cell apoptosis induced by radiation, reduction of the DNA damage repair caused by radiation, and reversion of epithelial mesenchymal cell transformation induced by radiation. RET regulates andcontrols the radiosensitivity of the tumor cells by regulating PTEN. Under radiation pressure, PTEN can reverse the tumor cell growth, apoptosis increasing, DNA damage repair and EMT generation induced by RET silencing, thereby regulating the tumor cell radiosensitivity induced by RET.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of LncRNA RET in regulating radiosensitivity of tumor cells. Background technique [0002] Genome-wide transcriptome studies have shown that there are a large number of non-coding RNAs (ncRNAs). Non-coding RNAs (non-coding RNAs, ncRNAs) refer to RNA molecules that are not translated into proteins in the transcriptome, including microRNAs (microRNAs, miRNAs), Small nuclear RNA (snRNA), nucleolar small RNA (small nucleolarRNAs, snoRNA), interfering small RNA (small interfering RNA, siRNA), RNA interacting with piwi protein (piwi-interacting RNA, piRNA) etc., long non-coding RNA (long non-codingRNA, LncRNA) and so on. Recent studies estimate that the number of lncRNAs in the human body is about 15,000, and the expression patterns of most lncRNAs are found to be tissue-specific. According to the location and characteristics of LncRNA, it can be divided into five types: ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K41/00A61K31/7088A61P35/00
CPCA61K31/7088A61K41/0038A61K45/00A61P35/00
Inventor 顾永清周平坤李雪萍朱茂祥王治东杨陟华刘晓丹
Owner ACADEMY OF MILITARY MEDICAL SCI
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