A kind of method of hybrid combination of celery

Abstract

The invention discloses a method for hybridization of celery. It first obtains several genetically stable cytoplasmic male sterile lines (CMS) of celery through transgenics, uses these CMS lines as female parents, selects and determines an excellent inbred line as male parent, carefully manages them in winter, and cultivates them into robust Seed the plants and complete the low-temperature vernalization process. In spring, several CMS female parents and corresponding male parents are planted in a seed collection bed at the same time according to the hybrid combination plan. Isolate from outside pollen, and then use low-density isolation mesh bags to block flower branches of all CMS female plants in the net room. Artificial assisted pollination was carried out during the flowering period. Hybrid seeds can be harvested from the CMS female parent plant when the seeds turn yellow. The method of the invention realizes the hybrid combination of one male parent and multiple CMS female parents at the same time, obtains sufficient hybrid seeds, improves hybrid breeding efficiency and pest control effect, and facilitates field management.

Description

technical field [0001] The invention belongs to the technical field of vegetable planting, and relates to a method for hybridization and assembly of celery. Background technique [0002] Celery is a biennial vegetable of the Umbelliferae genus Celery. It is an insect-borne flower and is often cross-pollinated. The flowers are small and large, and the flowering branches are soft and overlapping. The flowering period is long and scattered. This special flowering habit makes it difficult for celery to undergo strict artificial emasculation and cross-pollination. Make the celery hybrid breeding work can not realize the breakthrough progress for a long time. The application of the male sterile line of celery has solved this difficult problem well, and opened up an effective way for the work of cross breeding of celery. Chinese invention patent ZL201010300772.9 "Method for Propagation and Hybrid Seed Production of Celery Recessive Nuclear Gene Male Sterile Line" reproduces celery...

AI Technical Summary

Problems solved by technology

Chinese invention patent ZL201010300772.9 "Method for Propagation and Hybrid Seed Production of Celery Recessive Nuclear Gene Male Sterile Line" reproduces celery recessive nuclear gene male sterile line through asexual reproduction and realizes the goal of celery hybrid seed production, but the The method and technology are relatively complicated, and large-scale hybrid seed production has certain limitations

Chinese invention patent ZL201410705174.8 "A method and application of cross-pollination of celery" provides a cross-pollination method for cytoplasmic male sterility (CMS) of celery, so that the CMS female parent plants in the celery hybrid combination can obtain parent While this plant is effectively pollinated, 100% pollen isolation is achieved between different combinations, but the breeding efficiency of the one-to-one hybrid pollination and isolation of celery parent plants determined by this method is relatively low, and the pollination effect is poor. Problems such as less, timely and incomplete control of diseases and insect pests, and inconvenient field management need to be solved through technological innovation