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Application of lotus leaf alcohol extract in preparation of drug for inhibiting cancer metastasis

A technology for inhibiting cancer and alcohol extract, applied in the field of biomedicine, can solve the problems of lack of treatment strategy, difficult treatment, recurrence and metastasis of patients, etc., and achieve significant social and economic benefits, inhibit cancer metastasis, and have significant therapeutic effects.

Inactive Publication Date: 2018-12-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, metastatic cells are often more resistant to anticancer therapies, which makes them more difficult to treat
[0004] Although standardized comprehensive treatment methods such as surgery, radiotherapy and chemotherapy, and new strategies such as targeted therapy and immunotherapy have achieved certain curative effects on patients with recurrence and metastasis, due to their particularity and complexity, as well as basic research, clinical application research and medical treatment Due to technical limitations, there is still a lack of treatment strategies for how to prevent recurrence and metastasis in patients

Method used

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  • Application of lotus leaf alcohol extract in preparation of drug for inhibiting cancer metastasis
  • Application of lotus leaf alcohol extract in preparation of drug for inhibiting cancer metastasis
  • Application of lotus leaf alcohol extract in preparation of drug for inhibiting cancer metastasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: preparation lotus leaf alcohol extract

[0027] According to the ratio of lotus leaf powder in grams to ethanol aqueous solution in milliliters of 1:15, let the dried lotus leaf powder and the concentration of 70% ethanol aqueous solution in volume be mixed evenly, and then shake and extract at a temperature of 35°C and a rotation speed of 200rpm After 12 hours, filter and separate, the residue obtained was repeatedly extracted 2 times in the same manner, the residue was discarded, and the filtrate was combined; Under the conditions of a pressure of 400 mm Hg and a rotation speed of 50 r / min, rotary evaporation was carried out to dryness to obtain the ethanol extract of lotus leaf.

Embodiment 2

[0028] Embodiment 2: CCK8 cell proliferation-toxicity experiment

[0029] The obtained ethanol extract of lotus leaf was dissolved in DMSO to prepare ethanol extract of lotus leaf with different concentrations of 10, 25, 50, 100, and 250 μg / ml for cell experiments. Experimental group settings: different concentrations of lotus leaf alcoholic extract (respectively 10, 25, 50, 100, 250 μg / ml of lotus leaf alcoholic extract); control group: adding an equal volume of dimethyl sulfoxide (DMSO). The MDA-MB-231 breast cancer cells in the logarithmic growth phase were used to prepare cell suspensions, and then uniformly inoculated into 96-well plates at a seeding density of 4000-5000 cells per well. After 12 hours, the cells adhered to the wall, and were stimulated by adding drugs according to the set control group and experimental group, and 6 replicate wells were set for each concentration gradient. After 48 hours, 10 μl CCK8 was added to each well, and the color was developed in a...

Embodiment 3

[0031] Embodiment 3: cell scratch experiment

[0032] Inoculate the cells in a six-well plate. After the cells are full, use a pipette tip with a capacity of 200 microliters to make a scratch of uniform thickness in the middle of each six-well plate, and wash it twice with PBS. Add 2ml of serum-free medium to the well, and add the corresponding concentration of ethanol extract of lotus leaf, cultivate for 0, 24, and 36 hours, and take pictures under the microscope to record the results. figure 2 50μg / ml and 250μg / ml of lotus leaf ethanol extract on cancer cell metastasis rate, C is the control group. It can be seen that the ethanol extract of lotus leaf at 250 μg / ml can reduce the metastasis rate of cancer cells by about 87.5%. It shows that the lotus leaf alcohol extract can significantly inhibit the metastasis of cancer cells, and the ability to inhibit cancer cell metastasis increases with the increase of the concentration of the lotus leaf alcohol extract, which is conce...

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Abstract

The invention discloses an application of a lotus leaf alcohol extract in preparation of a drug for inhibiting cancer metastasis and belongs to the technical field of biomedicine. Mice suffering frommetastatic cancer are used as experimental subjects, compared with a control group fed with normal feed, the mice which suffer from the metastatic cancer and are fed with feed containing the lotus leaf alcohol extract have obviously reduced weight and have the lung metastasis number reduced by about 45% and the total tumor weight at the metastasis part reduced by about 50%. The fact that the lotusleaf alcohol extract can be used for effectively inhibiting cancer metastasis is put forward for the first time, so that a new way is opened up for treatment of metastatic tumor, great significance is made in the development of edible and medicinal dual-purpose resources, high-value conversion of active plant resources is realized, and deep processing and utilization of agricultural products is promoted.

Description

technical field [0001] The invention relates to the application of lotus leaf ethanol extract in the preparation of drugs for inhibiting cancer metastasis, and belongs to the technical field of biomedicine. Background technique [0002] Lotus leaf is the leaf of Nelumbo nucifera Gaertn (Nelumbo nucifera Gaertn), also known as lotus leaf and lotus leaf. Medicine plants. Pharmacological and pharmacological studies on lotus leaf at home and abroad show that lotus leaf has the functions of regulating fat, reducing weight, anti-oxidation, antibacterial, lowering blood pressure and protecting vascular endothelium, but its application in the field of cancer has not been reported. [0003] Cancer is a disease caused by the loss of normal regulation and excessive proliferation of body cells. At present, it has become one of the leading causes of death in developed countries, and the final result of cancer development is systemic cancer cell metastasis throughout the body, which eve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/62A61P35/04
CPCA61K36/62A61K2236/333A61K2236/51A61K2236/53A61P35/04
Inventor 何钊童跃林
Owner JIANGNAN UNIV
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