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Human placenta mesenchymal stem cell transporting liquid

A technology for transporting liquid and stem cells, which is applied in the field of stem cells, can solve the problems of reduced number of viable cells, unsuitable for long-distance transportation, etc., and achieve the effect of improving the survival rate

Active Publication Date: 2019-01-04
深圳华大基因细胞科技有限责任公司
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Problems solved by technology

However, the number of viable cells of the three types of cells (human amniotic fluid, umbilical cord, and placenta-derived mesenchymal stem cells) decreased significantly after being placed in the cell transport solution at room temperature for 72 hours, which is not suitable for long-distance transport

Method used

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Experimental program
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Embodiment Construction

[0014] 1. Experimental materials

[0015] Choose fresh placentas from healthy puerperas, sign the informed consent form, and agree to be used in scientific research experiments.

[0016] Human placental mesenchymal stem cell culture medium was purchased from Saiye Company; fetal bovine serum was purchased from American Thermo Company; DMEM medium was purchased from American Gibco Company; The culture medium was purchased from Shanghai Raas Blood Products Co., Ltd.; the stem cell osteogenic differentiation medium was purchased from PromoCell; the neural differentiation medium: 20 μg / L epidermal growth factor and 20 μg / L were added to the DMEM / F12 medium containing 2% B27 Formulated with Basic Fibroblast Growth Factor of L.

[0017] 2. Experimental method

[0018] 1. Isolation and culture of human placental mesenchymal stem cells (hPMSCs)

[0019] Peel off the amniotic membrane, take the placental tissue from the fetal face, cut it into pieces, wash it several times with PBS ...

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Abstract

The invention relates to human placenta mesenchymal stem cell transporting liquid. Adding 5micron heterophyllin C on the basis of the prior art effective overcomes the defect of evident cell viabilitylowering after suspension of the transporting liquid for 72h in the prior art. The transporting liquid is prepared from, by mass, 30% of 0.2% trehalose solution (PBS solution of trehalose), 25% of red blood cell storage liquid, 5% or 0.2% heparin calcium solution (PBS solution of heparin calcium) and 40% of human albumin injection by well mixing at the normal temperature and contains 5micron heterophyllin C. The transporting liquid has the advantage that viability of human placenta mesenchymal stem cells in transporting for 72h is improved without affecting the multi-directional differentiation performance.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a human placenta mesenchymal stem cell transport solution. Background technique [0002] Stem cells are a class of cells with self-renewal and multilineage differentiation capabilities. It is because of its regenerative potential and plasticity that it is widely used in regenerative medicine and stem cell research. Stem cells offer hope for many diseases that currently lack effective treatments, including stroke, Huntington's disease, Alzheimer's disease and Parkinson's disease. [0003] Mesenchymal stem cells are multipotent stromal cells isolated from adult and fetal tissues, defined as adherent fibroblast-like cells. Mesenchymal stem cells have intrinsic homing ability, can migrate to damaged tissues, and actively participate in tissue repair. Although mesenchymal stem cells are pluripotent, their self-renewal ability gradually decreases leading to stagnation of cell division, and...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 卢珊珊郑桂纯
Owner 深圳华大基因细胞科技有限责任公司
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