Clinical-grade serum-free cell cryopreservation solution

A cryopreservation liquid and serum-free technology, which is applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of low cell recovery rate, increased pollution, complex components, etc., and achieve simplified cryopreservation procedures and adaptability Widespread, recovery-enhancing effects

Inactive Publication Date: 2019-01-04
YOCON BIOLOGY TECH CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] When the cell cryopreservation solution is used to preserve the cells, the cells are protected by the cryoprotectant during the cryopreservation process. The traditional cell protectant is only an intracellular protectant. The cells are easily damaged by ice crystals during cryopreservation, and the recovery rate of the cells is low. inability to effectively protect cells
In addition, the cell cryopreservation solution in the prior art usually contains animal-derived substances with complex components, which has potential risks in clinical application and increases the chance of contamination. At the same time, most of the cryopreservation solutions currently contain DMSO (dimethyl sulfoxide), there is a certain toxicity in clinical application

Method used

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  • Clinical-grade serum-free cell cryopreservation solution
  • Clinical-grade serum-free cell cryopreservation solution
  • Clinical-grade serum-free cell cryopreservation solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Preparation of cell cryopreservation solution

[0063] It is composed of components with the following concentrations, and the units of each concentration are mg / L unless otherwise specified:

[0064] L-Arginine 200;

[0065] CuSO 4 ·5H 2 O 0.001;

[0066] L-Asparagine 50;

[0067] L-aspartic acid 20;

[0068] L-glutamic acid 20;

[0069] Ni (NO 3 ) 2 · 6H 2 O 0.0001;

[0070] L-Glutamine 250;

[0071] ZnSO 4 · 7H 2 O 0.3;

[0072] Glycine 10;

[0073] CoCl 2 · 6H 2 O 0.004;

[0074] L-histidine 20;

[0075] NaSiO 3 · 9H 2 O 0.005;

[0076] L-Isoleucine 50;

[0077] Na 3 VO 4 · 12H 2 O 0.002;

[0078] L-lysine hydrochloride 40;

[0079] SnC1 2 · 2H 2 O 0.00005;

[0080] L-methionine 20;

[0081] Na 2 SeO 3 0.006;

[0082] L-phenylalanine 20;

[0083] FeSO 4 · 7H 2 O 1.0;

[0084] L-proline 20;

[0085] Glucose 2500;

[0086] L-serine 30;

[0087] Vitamin C 0.500;

[0088] L-threonine 20;

[0089] Paraben 1.0;

[0090]...

Embodiment 2

[0103] Example 2 Preparation of cell cryopreservation solution

[0104] It is composed of components with the following concentrations, and the units of each concentration are mg / L unless otherwise specified:

[0105] L-Arginine 100;

[0106] CuSO 4 ·5H 2 O 0.0005;

[0107] L-asparagine 75;

[0108] L-aspartic acid 30;

[0109] L-glutamic acid 10;

[0110] Ni (NO 3 ) 2 · 6H 2 O 0.00002;

[0111] L-Glutamine 500;

[0112] ZnSO 4 · 7H 2 O 0.6;

[0113] Glycine 5;

[0114] CoCl 2 · 6H 2 O 0.001;

[0115] L-histidine 30;

[0116] NaSiO 3 · 9H 2 O 0.01;

[0117] L-isoleucine 25;

[0118] Na 3 VO 4 · 12H 2 O 0.0005;

[0119] L-lysine hydrochloride 60;

[0120] SnC1 2 · 2H 2 O 0.0001;

[0121] L-Methionine 10;

[0122] Na 2 SeO 3 0.002;

[0123] L-Phenylalanine 30;

[0124] FeSO 4 · 7H 2 O 0.16;

[0125] L-proline 10;

[0126] Glucose 1000;

[0127] L-serine 45;

[0128] Vitamin C 0.704;

[0129] L-threonine 10;

[0130] p-hydroxybenzoic ac...

Embodiment 3

[0143] Example 3 Preparation of cell cryopreservation solution

[0144] It is composed of components with the following concentrations, and the units of each concentration are mg / L unless otherwise specified:

[0145] L-Arginine 300;

[0146] CuSO 4 ·5H 2 O 0.005;

[0147] L-asparagine 25;

[0148] L-Aspartate 10;

[0149] L-glutamic acid 30;

[0150] Ni (NO 3 ) 2 · 6H 2 O 0.0002;

[0151] L-Glutamine 100;

[0152] ZnSO 4 · 7H 2 O 0.06;

[0153] Glycine 15;

[0154] CoCl 2 · 6H 2 O 0.008;

[0155] L-histidine 10;

[0156] NaSiO 3 · 9H 2 O 0.001;

[0157] L-isoleucine 75;

[0158] Na 3 VO 4 · 12H 2 O 0.005;

[0159] L-lysine hydrochloride 20;

[0160] SnC1 2 · 2H 2 O 0.00001;

[0161] L-methionine 30;

[0162] Na 2 SeO 3 0.01;

[0163] L-Phenylalanine 10;

[0164] FeSO 4 · 7H 2 O 0.2;

[0165] L-proline 30;

[0166] Glucose 4000;

[0167] L-serine 15;

[0168] Vitamin C 0.176;

[0169] L-threonine 30;

[0170] p-Hydroxybenzoic acid 1....

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Abstract

The invention discloses a serum-free cell cryopreservation solution which comprises an amino acid component, an inorganic salt component, an extracellular cryoprotectant, an intracellular cryoprotectant and the like. The cell cryopreservation solution disclosed by the invention has the advantages that double protective components are contained and a cell is simultaneously protected from ice crystal damage during cryopreservation intracellularly and extracellularly, so that the recovery rate of the cell is greatly increased; the cell cryopreservation solution is suitable for extensive cell linepreservation, can be used for long-term preservation of cells and can be applied to establishment of cell banks, scientific cell line preservation and long-term preservation of clinical mesenchymal stem cells, monocytes, immune cells and all mammalian cells involved; and meanwhile, the cell cryopreservation solution is a completely serum-free, protein-free and dimethylsulfoxide (DMSO)-free cryopreservation solution, so that heterologous exogenous components introduced in clinical applications are avoided and the solution is safer.

Description

technical field [0001] The invention relates to a clinical level serum-free cell cryopreservation solution. Background technique [0002] When the cell cryopreservation solution is used to preserve the cells, the cells are protected by the cryoprotectant during the cryopreservation process. The traditional cell protectant is only an intracellular protectant. The cells are easily damaged by ice crystals during cryopreservation, and the recovery rate of the cells is low. Cannot effectively protect cells. In addition, the cell cryopreservation solution in the prior art usually contains animal-derived substances with complex components, which has potential risks in clinical application and increases the chance of contamination. At the same time, most of the cryopreservation solutions currently contain DMSO (dimethyl sulfoxide), there is a certain toxicity in clinical application. Contents of the invention [0003] In view of the above-mentioned prior art, the present inventi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 李海峰张莹莹
Owner YOCON BIOLOGY TECH CO
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