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Year-round multi-round generation-adding breeding technique of Stevia rebaudiana Bertoni

A stevia and technology technology, which is applied in the directions of seed and rhizome treatment, horticulture, and botanical equipment and methods, can solve the problems of high resource consumption and long breeding years, and achieves the goal of speeding up the breeding process, shortening the breeding years, and shortening the growth cycle. Effect

Inactive Publication Date: 2019-01-15
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to overcome the problems of long breeding period and high resource consumption in the existing stevia breeding technology, so as to provide a multi-round addition method of stevia anniversaries, from one ripening a year to three to four ripenings a year, Effectively speed up the breeding process, shorten the breeding period, avoid traveling between north and south, and save manpower and material costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The multi-round addition breeding technology of Stevia anniversary is carried out according to the following steps:

[0018] 1) Early germination of seeds: In mid-September 2016, the stevia high total glycoside content variety 'Zhongshan No. 8' and the high RA glycoside content variety 'Zhongshan No. 6' were cross-pollinated, and the inflorescences about 10 days after pollination were collected and brought back to the laboratory , select black plump and immature seeds, place them in a petri dish covered with double-layer moistened filter paper, cover them, and place them in a light incubator at 25-28°C for germination. After 7-10 days, the desired stevia can be obtained Seedlings, in order to ensure the vitality of the seedlings, keep an appropriate amount of water in the petri dish during the germination process.

[0019] Step 2) The first transplanting and seedling management: at the beginning of October 2016, a 5×5cm square flowerpot was filled with substrate (nutrie...

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PUM

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Abstract

The invention provides a year-round multi-round generation-adding breeding technique of Stevia rebaudiana Bertoni. Through the effective control of three stages including seed germination and transplanting, seedling management and flowering management, three-round or four-round generation addition of the Stevia rebaudiana Bertoni is achieved, the breeding process is effectively accelerated, the breeding cycle is shortened, at the same time, the controllable greenhouse culture technology is used, the cross-region travel is avoided, the time is saved, and the human and material costs are reduced. The method is applied to the backcross and hybridization breeding of the Stevia rebaudiana Bertoni, and the breeding efficiency of the Stevia rebaudiana Bertoni can be greatly improved, and the process of result application is accelerated.

Description

technical field [0001] The invention relates to the technical field of plant breeding methods, in particular to a Stevia anniversary multi-round addition breeding technology. Background technique [0002] Stevia (Stevia rebaudiana Bertoni) belongs to the genus Asteraceae and is an emerging sugar crop. The steviol glycosides contained in its leaves have high sweetness and low calories (more than 300 times sweeter than sucrose, and only 1 / 300 of sucrose) It is a natural low-calorie sweetener, widely used in food, beverage, medicine, chemical industry and other fields. At present, my country is the world's largest stevia planter and exporter of steviol glycosides and raw materials. The selection and breeding of new stevia varieties is the basis for improving the yield and quality of stevia. Therefore, it is urgent to speed up the breeding process of stevia, and additional generation breeding is an important way to shorten the breeding period and speed up the breeding process. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/00A01C1/00
CPCA01C1/00A01G22/00
Inventor 杨永恒徐晓洋孙玉明张永侠张婷侯孟兰王银杰刘清泉原海燕
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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