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APOA4 protein-based gastric cancer detection kit and using method thereof

An APOA4 and detection kit technology, applied in the field of biomedicine, can solve the problems of unfavorable grassroots popularization, complicated operation, high cost of gastric cancer detection technology, and achieve the effect of good clinical application prospect, good sensitivity and high affinity.

Inactive Publication Date: 2019-01-15
李玉民
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The technical problem to be solved by the present invention is: the existing gastric cancer detection technology has high cost and complex operation, which is not conducive to popularization at the grassroots level. The present invention provides a gastric cancer detection kit based on APOA4 protein to solve the above problems

Method used

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  • APOA4 protein-based gastric cancer detection kit and using method thereof
  • APOA4 protein-based gastric cancer detection kit and using method thereof
  • APOA4 protein-based gastric cancer detection kit and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0047] 1. Clinical trial case data

[0048] The peripheral blood samples of 27 patients with gastric cancer and 13 normal controls (peripheral blood samples of normal patients without gastric cancer) were selected. The basic information is shown in Table 1.

[0049] Table 1 Basic information of clinical trials

[0050] Basic Information

Gastric cancer patient blood sample

Normal control blood samples

Number of people / case

27

13

age

30～60

30～60

male to female ratio

2:1

2:1

[0051] 2. Composition of gastric cancer detection kit based on APOA4 protein

[0052] A gastric cancer detection kit based on the APOA4 protein is given, as shown in Table 2.

[0053] Table 2 Composition of gastric cancer detection kit based on APOA4 protein

[0054]

[0055]

[0056] 3. Preparation of gastric cancer detection kit based on APOA4 protein

[0057] Step 1, serum sample preparation: collect peripheral blood with disposable non...

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Abstract

The invention discloses an APOA4 protein-based gastric cancer detection kit. The detection kit comprises a solution A, a solution B, a solution C, a solution D, a solution E, a solution F and a solution G, wherein in the solution A, Human APOA4 proteins are diluted into a working solution by adopting an antibody diluent to be used as a standard substance; in the solution B, a concentrated biotin detection antibody is diluted into a working solution by adopting a biotin detection antibody diluent, and a mouse-anti human AMY1A monoclonal antibody is adopted in the concentrated biotin detection antibody; in the solution C, a concentrated HRP compound is diluted into a working solution by adopting an HPR conjugated diluent; in the solution D, a rabbit-anti human AMY1A polyclonal antibody is diluted into a working solution by adopting an antibody diluent; the solution E is Wash Buffer; the solution F is a developing solution; and the solution G is a stop solution. The APOA4 protein-based gastric cancer detection kit is used for detecting the expression level of an APOA4 protein in serum by adopting ELISA method detection, the cost is lower, the operation is convenient, and basis popularization, generalization and application are facilitated.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an APOA4 protein-based gastric cancer detection kit and a method for using the same. Background technique [0002] Gastric cancer (Gastric Cancer) is one of the tumors with the highest incidence rate in the world. How to improve the early diagnosis and treatment of gastric cancer patients is a hot issue in domestic research. Because early gastric cancer has no obvious clinical manifestations, and the early clinical manifestations of gastric cancer are often confused with gastritis, so most patients with gastric cancer do not have specific clinical symptoms even if the tumor develops to an advanced or late stage. Therefore, it is obvious to rely on clinical manifestations to diagnose gastric cancer. can not achieve. Although gastroscopy screening has become a physical examination item for people above middle age, it is not widely accepted by ordinary people due to its invasiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/58G01N33/535
CPCG01N33/535G01N33/574G01N33/57484G01N33/581G01N33/68G01N2800/06
Inventor 李玉民赵阳
Owner 李玉民
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