Tissue culture technique of Chinese knotweed herb

A technology of tissue culture and fotan mother, which is applied in the field of plant tissue culture, can solve problems such as unfavorable protection of high-quality germplasm resources, restrictions on the promotion and planting of high-quality fiot mother, and separation of offspring traits of seed reproduction, so as to achieve the effect of accelerating promotion and resource development and utilization

Inactive Publication Date: 2019-01-18
林登淞
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, Fotanmu mainly uses seeds for seedling propagation, but due to the separation of offspring traits in seed propagation, i...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) Induction of clustered buds: select the budding stems of young trees that grow vigorously and have no diseases and insect pests in the current year as explants, soak them in an aqueous solution of detergent for 8 minutes, then rinse them with tap water for 13 minutes, dry the surface moisture and set aside. Soak in 75% ethanol for 8s in ultra-clean workbench, sterilize with 0.1% mercuric chloride solution for 11min, wash with sterile water for 6 times, dry and cut into 1.5cm-long stems and inoculate them on the induction medium. Cultivate in complete darkness at 28°C for 18 days, then place in light for 13 hours a day, light intensity 1500x, and culture temperature 27°C for 42 days to induce the formation of clustered buds, and the induction rate is 69%. The induction medium is MS+5mg / L6-BA+1.5mg / L NAA+0.2mmol / L La(NO 3 ) 2 +2.3% sucrose+0.6% agar+0.08% activated carbon, the pH value is 5.9.

[0013] (2) Proliferation culture: Transfer the clustered buds obtained ...

Embodiment 2

[0017] (1) Induction of clustered buds: Select the budding stems of young trees that grow vigorously and have no diseases and insect pests in the current year as explants, soak them in an aqueous solution of detergent for 10 minutes, then rinse them with tap water for 17 minutes, dry the surface moisture and set aside. Soak in 78% ethanol for 12s in an ultra-clean workbench, sterilize with 0.1% mercuric chloride solution for 12min, wash with sterile water for 6 times, dry and cut into 1.4cm-long stems and inoculate them on the induction medium. Cultivate in total darkness at 27°C for 24 days, then place in light for 16 hours a day, with light intensity of 1700x, and culture temperature at 27°C for 38 days to induce the formation of clustered buds, with an induction rate of 80%. The induction medium is MS+7mg / L6-BA+1.0mg / L NAA+1.3mmol / L La(NO 3 ) 2 +2.3% sucrose+0.5% agar+0.08% activated carbon, the pH value is 5.7.

[0018] (2) Proliferation culture: Transfer the clustered b...

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PUM

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Abstract

The invention discloses a tissue culture technique of Chinese knotweed herb. The Chinese knotweed herb belongs to polygonaceae plants, is mainly produced in Guangdong, Gangxi, Sichuan, Fujian and thelike, can be harvested all year round, and is neutral in nature, and sour and slightly astringent in taste. The Chinese knotweed herb has the effects of clearing away heat and toxic material, removingdampness by diuresis, relieving itching, improving acuity of vision and removing nebula, and is used for treating dysentery, diarrhea, jaundice and swelling and pain in throat. The tissue culture technique of the Chinese knotweed herb is a seedling raising method capable of obtaining high-quality seedlings by a rapid propagation in vitro technique. The tissue culture technique takes the stems with buds of the Chinese knotweed herb as explants, establishes a tissue culture and rapid propagation system of the Chinese knotweed herb by a process of explant disinfection, induction of multiple shoots, rooting culture, seedling hardening and transplantation and the like, and is of important practical significance in accelerating popularization and resource development and utilization of high-quality seedlings of the Chinese knotweed herb.

Description

technical field [0001] The invention relates to a method for plant tissue culture in plant biotechnology, in particular to a technique for tissue culture of the mother of charcoal. Background technique [0002] Fotan is a plant of the Polygonaceae family. It is mainly produced in Guangdong, Guangxi, Sichuan, Fujian and other places. It can be harvested throughout the year. It has a flat, sour and slightly astringent taste. It has heat-clearing and toxic substances removing, promoting dampness and relieving itching, improving eyesight and removing nebula. For dysentery, diarrhea, jaundice, sore throat. In recent years, the development of Fo Tan mother resources has reached a certain scale, and GAP planting bases have been established in Guangdong, Guangxi, Sichuan, Fujian, etc., which have played a certain role in promoting the protection of Fo Tan mother wild resources. At present, the seedlings of Fotanmu are mainly propagated by seeds, but the separation of offspring tra...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 林登淞
Owner 林登淞
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