Trichoderma longiflorum, solid microbial agent thereof and application thereof

A microbial inoculum, Trichoderma longbranchus technology, applied in microorganisms, microorganisms, microorganism-based methods, etc., can solve the problems of slow degradation speed and poor low temperature adaptability, and achieve rapid decomposing, convenient transportation and plant growth. Effect

Active Publication Date: 2019-01-18
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of slow degradation rate and poor low temperature adaptability of existing straw decomposing

Method used

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  • Trichoderma longiflorum, solid microbial agent thereof and application thereof
  • Trichoderma longiflorum, solid microbial agent thereof and application thereof
  • Trichoderma longiflorum, solid microbial agent thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Primary screening of Trichoderma longibrachiata

[0037]The surface soil samples were collected from the grassland of Hulunbeier, Inner Mongolia, and the strains were isolated by using the method of dilution and plate plating, and the PDA medium was used to isolate 129 Trichoderma strains with different colony forms. The isolated bacteria were streaked and purified several times to obtain Strains were purely cultured and preserved. Spot the purified 29 strains on sodium cellulose-Congo red medium and culture them at 16°C and 30°C respectively. Measure the colony diameter (d) and transparent circle diameter (D) after culturing for 5 days, and calculate D / d value.

[0038] Trichoderma longibrachiatum (Trichoderma longibrachiatum) with the preservation number CGMCC No.16280 can produce a transparent circle with a diameter of 2.2 cm or more on the sodium cellulose-Congo red medium, and the D / d value is the highest at 16°C and 28°C. 2.2 and 2.4 respectively, indicating...

Embodiment 3

[0045] Present embodiment provides a kind of Trichoderma longibrachiae solid inoculum, its preparation method comprises the steps:

[0046] Inoculate Trichoderma longibrachiatum with the preservation number CGMCC No.16280 on a PDA plate for activation, and culture at 28°C for 48 hours; inoculate 5 to 10 pieces of activated Trichoderma longibrachiae into PD liquid medium for seed cultivation, 28 Cultivate on a shaking table at 200 rpm for 48 hours; inoculate Trichoderma elongatum into Trichoderma solid medium with 5% inoculum amount, humidity 60%, carry out expansion culture on a shallow plate at room temperature for more than 5 days, and dry it to obtain long branch Trichoderma Mold agent.

[0047] The number of live bacteria in the microbial agent is 2×10 8 cfu~1×10 10 cfu / g.

Embodiment 4

[0049] The present embodiment provides a kind of composite microbial inoculum agent, comprises Trichoderma longibrachiae, Bacillus licheniformis and Streptomyces that preservation number is CGMCC No.12680, the ratio of the number of viable bacteria of described Trichoderma longibrachiae, Bacillus licheniformis and Streptomyces It is 3~5:1~2:1~2.

[0050] Its preparation method comprises the following steps:

[0051] Step 1. Inoculate Trichoderma longibrachiata with the preservation number CGMCC No.16280 on a PDA plate for activation, and culture at 28° C. for 48 hours; inoculate 5 to 10 pieces of activated Trichoderma into PD liquid medium for seed cultivation, Cultivate on a shaker at 28°C and 200rpm for 48 hours; inoculate Trichoderma longibrachiata into Trichoderma solid medium according to 5% inoculation amount, humidity 60%, carry out expansion culture in a shallow plate at room temperature, and dry in the air after 5 days to obtain Trichoderma longibranchii pink;

[00...

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Abstract

The invention relates to the technical field of industrial microorganisms, in particular to a Trichoderma longiflorum, a solid microbial agent thereof and an application thereof. The Trichoderma longibrachiatum (JG9-31, deposit No. CGMCC No. 16280. The invention provides Trichoderma longum JG9-31 can secrete cellulases, promote the rapid maturation of straw, can rapidly propagate in the low temperature range of 10-35 DEG C, and still has a strong ability to degrade cellulose under the low temperature condition, and can rapidly degrade straw. The invention also provides a solid microbial agent,comprising Trichoderma longiflorum JG9-31, Bacillus licheniformis and Streptomyces sp., three kinds of bacteria and the biological enzymes secreted by them form an efficient synergistic system, so that the compound microbial inoculum composed of the three kinds of bacteria can realize the rapid return of straw to the field under the low temperature condition without adding additional cellulase preparation into the compound microbial inoculum.

Description

technical field [0001] The invention relates to the technical field of industrial microorganisms, in particular to a kind of Trichoderma longibrachiata, its solid microbial agent and its application. Background technique [0002] Crop straw is one of the most abundant substances in the world. At present, the solution to the problem of large areas of straw in my country mainly relies on direct return to the field. Straw returning is an effective way to make full use of nutrient resources in straw and improve soil. After returning to the field, the straw decomposes under the action of soil microorganisms. The cellulose, hemicellulose and lignin in the straw are converted into simple compounds that are easily absorbed by plants, and the other parts are converted into humus, thereby improving the physical and chemical properties of the soil. Improve soil fertility, which in turn increases crop yield and quality. Especially in the north, under the double cropping system of corn...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/20C05F11/08C12R1/885C12R1/10C12R1/465
CPCC05F11/08C12N1/14C12N1/20C12N1/145C12R2001/885
Inventor 黄媛媛黄亚丽马慧媛徐炳雪邢慧珍贾振华
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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