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Oleanolic acid glucuronyltransferase and coding gene and application thereof

A technology of oleanolic acid glucuronic acid and oleanolic acid, which is applied in the field of plant genetic engineering and biology, can solve the problem of not identifying glycosyltransferases, etc., and achieve the effect of promoting biosynthesis and increasing yield

Active Publication Date: 2019-01-25
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

[0004] Although oleanane-type saponins are also abundant in other Panax genus plants and their transcriptome information has been reported, no glycosyltransferases involved in the synthesis of oleanane-type saponins have been identified

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  • Oleanolic acid glucuronyltransferase and coding gene and application thereof
  • Oleanolic acid glucuronyltransferase and coding gene and application thereof
  • Oleanolic acid glucuronyltransferase and coding gene and application thereof

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Embodiment Construction

[0045] The present invention will be further described in detail below in conjunction with the examples.

[0046] Those skilled in the art will understand that the following examples are only for illustrating the present invention and should not be considered as limiting the scope of the present invention. If no specific technique or condition is indicated in the examples, it shall be carried out according to the technique or condition described in the literature in this field or according to the product specification. The materials or equipment used are not indicated by the manufacturer, and they are all conventional products that can be obtained through purchase.

[0047] In the present invention, unless otherwise stated, the percentage sign is a mass percentage.

[0048] To find oleanolic acid glycosyltransferase (OGAT) genes, the transcriptome data of Panax notoginseng and Panax notoginseng were searched for genes related to glycosylation, and these genes were compared wi...

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Abstract

The invention relates to an oleanolic acid glucuronyltransferase, a coding gene and an application thereof, belonging to the field of plant genetic engineering and biotechnology. The amino acid sequence of the oleanolic acid glucuronyltransferase of the present invention is shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 or SEQ ID NO.4. A nucleotide sequence is shown in SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7 or SEQ ID NO.8. The oleanolic acid glucuronyltransferase of the invention can specifically take uridine diphosphoglucuronic acid as a glycosyl donor and catalyze the glycosylation of oleanolic acid C-3 to produce oleanolic acid 3-O-beta-Glucuronic acid. This enzyme is the upstream step in the synthesis of oleanolic saponins, which is of great significance for the elucidation of the synthetic pathway of oleanolic saponins.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering and biotechnology, and specifically relates to oleanolic acid glucuronosyltransferase and its coding gene and application. Background technique [0002] Ginger-like Sanqi (Panax zingiberensis C.Y.Wu et K.M.Feng) is a perennial herb of the Araliaceae genus Ginseng, distributed in southeastern Yunnan, central Nepal, high-altitude areas of Bhutan, Taunggyi, Shan State and other places in Myanmar. Ginger-shaped Panax notoginseng rhizomes are like ginger lumps, which is an endangered species. Due to over-exploitation, wild resources are extremely scarce at present. Ginger-shaped Panax notoginseng has the effects of dispersing blood stasis, relieving pain, and stopping bleeding, and is mainly used for bruises, fractures, hematemesis, dysfunctional uterine bleeding, and traumatic bleeding. [0003] Saponins are the main active ingredients of ginger-like notoginseng, mainly containing dammarane-t...

Claims

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Application Information

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IPC IPC(8): C12N9/10C12N15/54C12P33/00C12P19/02
CPCC12N9/1051C12Y204/01017C12N15/70C12P19/00
Inventor 杨生超唐卿雁张广辉范伟陈庚
Owner YUNNAN AGRICULTURAL UNIVERSITY
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