Test method for expression of Ermiao powder on rheumatoid arthritis inflammation suppression factors

A test method and rheumatoid technology, applied in the medical field, can solve the problem of lack of scientific basis of Ermiao Powder, and achieve the effect of reducing the number of inflammatory cells and slowing down the proliferation of membrane tissue

Inactive Publication Date: 2019-02-01
SHANXI UNIV OF CHINESE MEDICINE
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Problems solved by technology

Lack of scientific basis for the pharmacological mechanism of Ermiao San in the treatment of rheumatoid arthritis

Method used

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  • Test method for expression of Ermiao powder on rheumatoid arthritis inflammation suppression factors
  • Test method for expression of Ermiao powder on rheumatoid arthritis inflammation suppression factors
  • Test method for expression of Ermiao powder on rheumatoid arthritis inflammation suppression factors

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Embodiment Construction

[0041] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0042] A kind of Er Miao powder is to rheumatoid arthritis anti-inflammatory factor expression test method, test material comprises 80 SD rats, normal saline, three kinds of doses of Er Miao powder water decoction, methotrexate, test method comprises the following steps:

[0043] Step 1: Handling of Test Animals

[0044]80 SD rats were used to establish the CIA model, with a body weight of (180±15) g, provided by the Experimental Animal Center of the Academy o...

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Abstract

The invention discloses a test method for expression of Ermiao powder on rheumatoid arthritis inflammation suppression factors. The test materials include: 80 SD rats, normal saline, three doses of Ermiao powder decoction and methotrexate. The method includes: conducting gastric perfusion of normal saline on the 80 SD rats, performing gastric perfusion of the three doses of Ermiao powder decoction, and carrying out gastric perfusion of methotrexate at a dosage of 2.7mg/(kg.week) to obtain a positive control group, performing 1mL/100g gastric perfusion each time for 15d continuously, collectingblood, conducting killing inspection, establishing a CIA rat model, and observing the spleen coefficient change and joint synovium section pathological change; and detecting the IL-6 and TNF-alpha level change of serum, the IL-6 and TNF-alpha level change of phagocytes, the expression of cholinergic anti-inflammatory pathway key receptor alpha 7nAChR and other indexes to discuss the RA treatmentmechanism. The result of the invention indicates that the Ermiao powder group joint cavity synovial tissue is villiform, stretches into the joint cavity and has few inflammatory cells, and Ermiao powder has a regulation effect on CHRNA7, thus providing a scientific basis for exploring the pharmacological mechanism of Ermiao powder in treatment of rheumatoid arthritis.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for testing the expression of Ermiao powder on the expression of anti-inflammatory factors in rheumatoid arthritis. Background technique [0002] Er Miao San is derived from "Danxi Xinfa". It is composed of atractylodes rhizome and Cortex Phellodendri in equal proportions. Cortex Phellodendron is bitter and cold to clear away heat, while Atractylodes atractylodis is bitter and warm to dry dampness. It is a traditional Chinese medicine classic for treating RA. At present, the studies of Ermiao Powder reported in the literature mainly focus on the content determination and drug efficacy of the single herb, and the effect of Ermiao Powder on joint tissue and serum inflammatory factors. Chen et al. confirmed that Ermiao Powder has immunosuppressive effect, among which Phellodendron Phellodendron is the main inhibitory drug, and berberine is the main immunosuppressive compon...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/756A61P19/02A61P29/00A61P37/04G01N33/50
CPCA61K36/284A61K36/756A61P19/02A61P29/00A61P37/04G01N33/5008A61K2300/00
Inventor 郝慧琴李振关建红王泽高玉亭
Owner SHANXI UNIV OF CHINESE MEDICINE
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