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A preparation method of monkfish skin peptide and the immunomodulatory application of the prepared monkfish skin peptide

A fish skin and health technology, applied in the field of monkfish skin peptides, can solve the problems of unsuitable monkfish skin peptides, inability to remove the fishy odor of marine fish, and large molecular weight of peptides, and achieves high fish skin utilization rate, uniform molecular weight, The effect of high immune activity

Active Publication Date: 2021-09-17
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The soft-shelled turtle peptide preparation method directly prepares polypeptides through enzymatic hydrolysis, and the obtained product has a high content of soft-shelled turtle protein, and the molecular weight of the polypeptide is relatively large. At the same time, this method cannot remove the fishy smell of marine fish, so it is not suitable for direct use in monkfish. Preparation of skin peptide

Method used

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  • A preparation method of monkfish skin peptide and the immunomodulatory application of the prepared monkfish skin peptide

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Effect test

Embodiment 1

[0030] A preparation method of monkfish skin peptide, comprising the following steps:

[0031] (1) Clean the fish skin scraps of the recovered monkfish, cut into pieces, then mix the fish skin with water, add vitamin E to disperse evenly, then add lipase to degrease at 30°C for 4 hours, and then add 25 wt% isopropyl Degrease the alcohol solution for 2 hours again, sieve the fish skin and rinse it with water until there is no smell of isopropanol, the amount of vitamin E added is 0.5wt% of the fish skin, the amount of lipase added is 1.6wt% of the fish skin, and the lipase activity is 30000U / g;

[0032] (2) Put the degreased fish skin into the water dispersed with high-temperature calcined mussel powder, immerse and deodorize, then sieve, rinse and recover the fish skin, wherein the high-temperature calcined mussel powder is made through the following process: after cleaning the mussel shells Grind it to 60-80 mesh, then mix it with sodium carbonate and silane coupling agent ...

Embodiment 2

[0044] A preparation method of monkfish skin peptide, comprising the following steps:

[0045] (1) Clean the recovered monkfish skin scraps, cut into pieces, then mix the fish skin with water, add vitamin E to disperse evenly, then add lipase to degrease at 38°C for 2 hours, then add isopropanol solution to degrease again 1h, sieve the fish skin and wash it with water until there is no smell of isopropanol, the amount of vitamin E added is 0.8wt% of the fish skin, the amount of lipase added is 1.2wt% of the fish skin, the lipase activity is 40000U / g, isopropyl Alcohol solution concentration 30wt%;

[0046] (2) Put the degreased fish skin into the water dispersed with high-temperature calcined mussel powder, immerse and deodorize, then sieve, rinse and recover the fish skin, wherein the high-temperature calcined mussel powder is made through the following process: after cleaning the mussel shells Grind it to 60-80 mesh, then mix it with sodium carbonate and silane coupling age...

Embodiment 3

[0053] A preparation method of monkfish skin peptide, comprising the following steps:

[0054] (1) Clean the reclaimed monkfish skin scraps, cut into pieces, then mix the fish skin with water, add vitamin E to disperse evenly, then add lipase to degrease 3 at 40°C, and then add 32wt% isopropanol The solution was degreased again for 3 hours, and the fish skin was sieved and rinsed with water until there was no smell of isopropanol. The amount of vitamin E added was 1 wt% of the fish skin, the amount of lipase added was 2 wt% of the fish skin, and the lipase activity was 50000U / g;

[0055] (2) Put the degreased fish skin into the water dispersed with high-temperature calcined mussel powder, immerse and deodorize, then sieve, rinse and recover the fish skin, wherein the high-temperature calcined mussel powder is made through the following process: after cleaning the mussel shells Grind it to 60-80 mesh, then mix it with sodium carbonate and silane coupling agent at a mass ratio o...

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Abstract

The invention relates to the technical field of monkfish skin polypeptides. Aiming at the problem that the polypeptides prepared from marine fishes have a relatively large molecular weight and a bitter smell, the invention provides a preparation method of monkfish skin peptides, which comprises making monkfish skin Lipase and isopropanol are combined to degrease, and the mussel powder is calcined at high temperature to remove the fishy smell, and the bitter smell of the immunomodulatory peptide is synergistically removed, and then the acidic environment, pulse electric field, and sodium borohydride and sodium acetate borohydride are synergistically weakened The degree of cross-linking between fish skin protein molecules strengthens the swelling of fish skin protein, and then separates and extracts fish skin protein with a protein extraction enzyme that has a weak decomposing effect on fish skin protein, and then uses a highly specific proteolytic enzyme to decompose fish skin Ankang fish skin peptide is made from protein, the molecular weight is 1000-3500Da, the specific gravity is high, compared with fish skin protein, the yield can reach about 78wt%, the molecular weight is uniform, and there is no bitter smell, the utilization rate of fish skin is high, and it has immune activity High, can be used in immune regulation.

Description

technical field [0001] The invention relates to the technical field of monkfish skin polypeptides, in particular to a method for preparing monkfish skin peptides and the immunoregulatory application of the prepared monkfish skin peptides. Background technique [0002] Polypeptides with immunomodulatory activity can participate in the regulation of specific and non-specific immune functions of the body by affecting the growth and development of immune organs, the activity and function of immune cells, and the expression, synthesis and release of immune molecules, and play an important role in maintaining the normal and healthy immune function of the body. effect. Generally, the smaller the molecular weight of the polypeptide, the higher the immunomodulatory activity. At present, the acquisition of polypeptides with immunomodulatory activity relies on chemical synthesis, DNA recombinant expression, and extraction from biological tissues and organs. Among them, marine biologi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34A23L33/18
CPCA23V2002/00A23L33/18C07K1/34C12P21/06A23V2200/324A23V2250/55
Inventor 杨最素
Owner ZHEJIANG OCEAN UNIV
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