Method for detecting toxicity of antibacterial agent
A detection method and technology for antibacterial agents are applied in the field of antibacterial agent toxicity detection, which can solve the problems of less results and achieve the effect of short experimental period and less instrument requirements.
Active Publication Date: 2019-02-12
UNIV OF SCI & TECH OF CHINA
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Problems solved by technology
However, the chronic toxicity of antibacterial agents is rarely studied, and triclocarban has less research results than other antibacterial agents
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[0042] The model organism is Caenorhabditis elegans. The nematodes were taken out from the -80°C refrigerator and thawed. When the nematodes developed to the third generation, the nematodes were lysed to obtain eggs. After incubation at 20°C for 24 hours, the L1 stage nematodes were obtained.
[0043] The configuration method of the triclocarban is as follows: use dimethyl sulfoxide (DMSO) as a solvent to configure a 1000 mg / L storage solution.
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Abstract
The invention provides a method for detecting the toxicity of an antibacterial agent. The method comprises the steps that caenorhabditis elegans is exposed in a to-be-detected sample, and according tothe body length, the body bending frequency, the head swing frequency, the offspring number, the incubation time, the generation time and / or the population scale of the caenorhabditis elegans, the toxicity of the antibacterial agent to growth and development, neural systems and / or reproductive systems is evaluated, wherein the to-be-detected sample contains the antibacterial agent. Compared withthe prior art, the method for detecting the toxicity of the antibacterial agent, provided by the invention, is rapid and efficient, has a short experiment period and few requirements on an instrument,and can estimate a safety application range of the antibacterial agent in daily care products; and meanwhile, the detection method provided by the invention provides a technical basis for speculatingthe influence of the antibacterial agent on offspring, and the influence of the antibacterial agent on parental nematodes and offspring of the parental nematodes can be obtained.
Description
technical field [0001] The invention belongs to the technical field of toxicity detection, in particular to a detection method for the toxicity of an antibacterial agent. Background technique [0002] Caenorhabditis elegans is a small, free-living organism that can survive in many parts of the world and feeds primarily on bacteria. As a common model organism, C. elegans has the advantages of short life cycle, compact genome, easy culture, small size, fast reproduction speed, and sensitivity to poisons. Most importantly, more than 40% of the genes in C. elegans are homologous to humans; and, among the 17 reported signaling pathways, 12 have been found in both C. elegans and humans. At present, it has been successfully applied to the study of biological effects in various fields, including genomics, cell biology, neurology and aging. From an anatomical point of view, the structure of the adult worm is simple, with about 1000 somatic cells. C. elegans is amenable to genetic ...
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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5014
Inventor 童中华崇汉娟
Owner UNIV OF SCI & TECH OF CHINA
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