Culture medium used for microorganism solid flux fermentation and preparation method and application thereof

Abstract

The invention relates to a culture medium used for microorganism solid flux fermentation and a preparation method and application thereof, and belongs to the field of solid culturing fermentation of industrial fermentation microorganisms. The cultural medium used for microorganism solid flux fermentation is in a piece shape; the culture medium is composed of solid components and water, and the solid components of the culture medium are prepared from, by weight, 70%-90% of bran, 5%-20% of bean pulp and 5%-15% of rice bran; the culture medium is applied to pore plate solid flux culturing of industrial fermentation microorganisms, and not only is the preparation and inoculating time of the culture medium saved, but also the high flux screening efficiency of solid pore plate culturing is improved. The uniform tableting manufacturing mode is adopted, the subpackaging operation error caused by workers in the culture medium weighing process is reduced, the contamination risk after boiling inthe culture subpackaging process is reduced, and the stability and parallelism of the solid high flux fermentation culturing of a pore plate are improved.

Description

technical field [0001] The invention relates to a culture medium and its preparation method and application, in particular to a culture medium for microbial solid-state flux fermentation and its preparation method and application. Background technique [0002] Solid-state fermentation is a common way for industrial fermentation microorganisms to carry out industrial production of enzyme preparations and secondary metabolites. At present, the research on solid-state fermentation of industrial fermentation microorganisms mainly focuses on substrate optimization and strain selection. Substrate optimization is to optimize and transform the solid-state fermentation process to improve product quality; strain selection is to conduct natural, chemical, and physical mutations on production strains to obtain products that can be improved in solid-state fermentation. Target strains with excellent traits of quality. However, due to the randomness of strain mutation and the cumbersome ...

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Problems solved by technology

But in the follow-up long-term application, the inventor found that the aseptic subpackaging of the multi-well plate medium during the solid-state culture of filamentous fungi seriously affects the application efficiency of the solid-state culture device

Since each well of the multi-well plate is connected as a whole, each well needs to be weighed separately, and the loading capacity of the well is also small, so it is necessary to be very careful in the process of subpackaging the medium to ensure that each well is subpackaged and cultivated. The consistency of the base and the prevention of contamination of bacteria, the operation of this aspect greatly affects the efficiency of the solid-state flux culture device

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