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Method for comprehensively inducing purplication of tea buds and leaves

A technology for tea tree buds and leaves, which is applied in the field of comprehensively inducing purple buds and leaves of tea trees, can solve the problems of long breeding cycle, heavy field workload, cumbersome breeding process, etc., and achieve the effect of solving the long breeding cycle

Inactive Publication Date: 2019-02-22
无锡市茶叶品种研究所有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, at present, the selection and breeding of tea tree purple varieties still rely on the traditional field system selection and expansion. Inconvenient factors such as long breeding cycle, cumbersome selection process, and heavy field workload have become long-term insurmountable drawbacks in breeding work. To a certain extent, it greatly limits the selection and breeding of purple tea varieties.

Method used

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  • Method for comprehensively inducing purplication of tea buds and leaves
  • Method for comprehensively inducing purplication of tea buds and leaves
  • Method for comprehensively inducing purplication of tea buds and leaves

Examples

Experimental program
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Effect test

Embodiment example 1

[0030] (1) Selection and hydroponics of tea tree branches

[0031] Select 10 strong branches of the Yingshuang tea tree variety with fresh young leaves and consistent growth, and use pure water for hydroponics for 1 day.

[0032] (2) Drought stress treatment

[0033] Drain the water from the tea seedlings in hydroponics, and place them in a natural state for 15 hours to carry out drought stress treatment.

[0034] (3) Ultraviolet light treatment

[0035] The branches after the drought stress treatment were placed in the ultraviolet light incubator of the model UVC-254, the ultraviolet light wavelength was 254nm, and the radiation intensity range was 1.1w / m 2 , the daily irradiation time is 4h, and the ultraviolet light treatment cycle is 5d.

[0036] (4) Sucrose solution treatment

[0037] Press 100ml / m on the leaf surface of tea tree branches 2 Spray 0.05g / L sucrose solution evenly, and then let it be in a natural state.

[0038] (5) Nutrient solution culture

[0039] ...

Embodiment example 2

[0047] (1) Selection and hydroponics of tea tree branches

[0048] Select 10 strong branches of Fuding tea tree variety with axillary buds and consistent growth, and use pure water for hydroponics for 1.5 days.

[0049] (2) Drought stress treatment

[0050] Drain the water from the tea seedlings in hydroponics, and place them in a natural state for 20 hours to carry out drought stress treatment.

[0051] (3) Ultraviolet light treatment

[0052] The branches after the drought stress treatment were placed in the ultraviolet light incubator of the model UVC-254, the ultraviolet light wavelength was 254nm, and the radiation intensity range was 1.2w / m 2 , the daily irradiation time is 4.5h, and the ultraviolet light treatment cycle is 5.5d.

[0053] (4) Sucrose solution treatment

[0054] Press 150ml / m on the leaf surface of tea tree branches 2 Spray 0.05g / L sucrose solution evenly, and then let it be in a natural state.

[0055] (5) Nutrient solution culture

[0056] Config...

Embodiment example 3

[0064] (1) Selection and hydroponics of tea tree branches

[0065] Select 10 strong branches of Xicha No. 10 tea tree variety with fresh young leaves and consistent growth, and use pure water for hydroponics for 2 days.

[0066] (2) Drought stress treatment

[0067] Drain the water from the tea seedlings in hydroponics, and place them in a natural state for 35 hours to carry out drought stress treatment.

[0068] (3) Ultraviolet light treatment

[0069] The branches after the drought stress treatment were placed in the ultraviolet light incubator of the model UVC-254, the ultraviolet light wavelength was 254nm, and the radiation intensity range was 1.9w / m 2 , the daily irradiation time is 5h, and the ultraviolet light treatment cycle is 6d.

[0070] (4) Sucrose solution treatment

[0071] Press 200ml / m on the leaf surface of tea tree branches 2 Spray 0.05g / L sucrose solution evenly, and then let it be in a natural state.

[0072] (5) Nutrient solution culture

[0073] C...

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Abstract

The invention particularly relates to a method for inducing purplication of new tea leaves by comprehensively utilizing the simulation of changes of a natural ecological environment. On the basis clarifying the regulation of synthesis and expression of purplication genes in tea buds and leaves, the changes of the natural environment is simulated, and the regulation mode of gene expression profilesof anthocyanin key enzymes in the tea buds and leaves is affected by regulating the light quality and the light intensity, and the expression of key enzyme genes for anthocyanin synthesis is promotedby low temperature induction; the content of nitrogen, phosphorus and potassium elements is controlled to induce up-regulation of camellia anthocyanin synthesis pathway structural genes, meanwhile, asucrose solution is sprayed on the leaf surface, proper drought stress is used for affecting the nitrogen synthesis in the leaves, the anthocyanin accumulation amount in the tea buds and leaves is thus increased, and the purplication of the tea buds and leaves appears.

Description

technical field [0001] The invention belongs to the field of tea tree cultivation and breeding, and in particular relates to a method for comprehensively inducing purple buds and leaves of tea trees. technical background [0002] Tea tree "Purple Bud" is a special tea tree germplasm resource, which is relatively common in group tea gardens. The purple color of tea trees is mainly due to genetic mutations caused by climate and seasonal changes. The combination of low temperature, light, fertilizer nutrients and other environmental factors Under the influence, the gene expression of anthocyanin synthase is up-regulated, and the chlorophyll in the buds and leaves is converted into anthocyanins at the same time. Studies have shown that the purple buds and leaves of tea trees are closely related to the accumulation of anthocyanins in the buds and leaves. Generally, the anthocyanins contained in the purple buds and leaves of tea trees account for about 0.5% to 1% of the dry matter...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G7/06A01G2/10
CPCA01G7/06A01G31/00A01G2/10
Inventor 徐琪
Owner 无锡市茶叶品种研究所有限公司
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