Culture bottle with indicating device

Abstract

The invention provides a culture bottle with an indicating device. The culture bottle comprises a bottle body and an indicating structure, wherein a first bottleneck is formed in the upper end of thebottle body; a second bottleneck is formed in a side face at the upper end of the bottle body; the bottom of the bottle body is conical to form a conical bottom centrifuge tube; a supporting screen plate is arranged inside the bottle body, and a magnetic rod is arranged at the upper end of the supporting screen plate; a needle tube used for sampling is arranged on the second bottleneck; the indicating structure comprises a rubber plug, an inner indicator tube and an outer indicator tube; the outer indicator tube is connected to the outer side of the inner indicator tube in a sleeving manner, and a sleeve used for connecting the outer indicator tube and the bottle body is arranged at the end, which is close to the rubber plug, of the outer indicator tube; scale indicators are arranged on the outer indicator tube and the inner indicator tube; and an end cover is arranged at the end, which is far away from the sleeve, of the outer indicator tube, and a hydrophobic membrane for ventilatingis arranged at the upper end of the end cover. Compared with the prior art, the culture bottle disclosed by the invention has the advantages of being simple in structure, convenient to sample, excellent in sealing property and low in cost.

Description

technical field [0001] The invention relates to a microorganism culture device, in particular to a culture bottle with an indicating device. Background technique [0002] The in vitro culture of cells and tissues has become an essential part of life research and practice. A wide variety of cell types are cultured, from viruses to bacteria and fungi, from human cells to animal cells and plant cells. Some cells and tissues can grow in suspension, but a significant fraction of mammalian cells require surface attachment. Therefore, in addition to having good transparency, non-toxicity and sterility, the culture flasks, culture plates and culture dishes used to provide a cell culture environment in vitro also need surface modification to enable them to adhere, divide and grow. However, when some current culture bottles are in use, sampling is troublesome, microbial growth monitoring is inconvenient to monitor, and false positive signals are prone to occur. When cells and tissu...

AI Technical Summary

Problems solved by technology

However, when some culture bottles are used today, it is troublesome to take samples, and the monitoring of microbial growth is inconvenient to monitor, and false positive signals are prone to appear.

When cells and tissues are cultured in culture flasks, gas will be generated. At this time, a device will be used to monitor the amount of gas generated. Some current detection devices are not accurate enough and the measurement is inaccurate. If the gas sensor is used, the input cost of the device is relatively high.

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