Autofocusing laser induced fluorescence spectrum detecting device and autofocusing laser induced fluorescence spectrum detecting method based on liquid lens

Abstract

The invention provides an autofocusing laser induced fluorescence spectrum detecting device and an autofocusing laser induced fluorescence spectrum detecting method based on a liquid lens. The detecting device comprises a light source emitter, a collimating lens and a narrow-band filtering sheet which are sequentially placed. A light source emitted by the light source emitter sequentially passes through the collimating lens and the narrow-band filtering sheet to generate an incident light source; the data information output end of a CCD (charge coupled device) is connected with the CCD data information input end processed by an information processing unit, and the adjustment information output end processed by the information processing unit is connected with the adjustment information input end of the liquid lens. The repeatability of detection results is guaranteed, and the measurement precision and the control flexibility are improved.

Description

technical field [0001] The invention relates to the technical field of spectral detection, in particular to a liquid lens-based automatic focusing laser-induced fluorescence spectral detection device and method. Background technique [0002] Laser Induced Fluorescence (LIF) spectroscopic detection is one of the most sensitive detection technologies at present, and its concentration detection limit reaches 10-9mol / L ~ 10-13mol / L, even for some fluorescent reagents with high fluorescence efficiency. Single Molecule Detection. [0003] Among them, confocal LIFD detection equipment can effectively improve detection sensitivity and signal-to-noise ratio, and has high detection sensitivity. The current confocal LIFD detection methods usually manually adjust the focus manually, and the detection repeatability is greatly affected by human factors. Or by moving the overall optical imaging system, changing the imaging object plane of the optical system to achieve focus position focu...

AI Technical Summary

Problems solved by technology

Although the adjustment process can be automated instead of manual, which expands the flexibility of use to a certain extent, but the adjustment of the imaging object plane by mechanical moving parts to achieve automatic focusing, the control flexibility and measurement accuracy are still limited.

At the same time, the instability of the movement of the mechanical structure will bring certain errors and noises, which will affect the focusing results

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