Light-control nano-particle assembly and preparation method and application thereof

Abstract

The invention discloses a light-control nano-particle assembly and a preparation method and application thereof, and belongs to the field of drug controlled release. The light-control nano-particle assembly contains nano-particles and a photo-responsive polymer, wherein the photo-responsive polymer is grafted on the surface of the nano-particles through coordination, interaction of subject and object or a covalent bond. A photo-responsive group is connected to a main chain or a side chain of the polymer. The light-control nano-particles and drug molecules are self-assembled to form the light-control nano-particle assembly for coating the drug molecules. In an illumination condition, photo-responsive molecules on the polymer chain are changed, so that a structure of the polymer chain is changed, and the nano-particle assembly is disassembled, therefore the drug molecules coated by the assembly are released, and light-control drug release is realized.

Description

technical field

[0001] The invention belongs to the field of drug controlled release, and more specifically relates to a light-controlled nanoparticle assembly and its preparation method and application. Background technique

[0002] Cancer (ie, malignant tumor) is one of the major diseases that endanger human health and life. Chemotherapy (i.e. chemotherapy), as one of the cancer treatment methods, has obstacles and toxic side effects to anticancer drugs due to the physical environment of the body, thus limiting the effect of chemotherapy to a large extent. In recent years, the rapid development of nano-drug delivery systems has greatly improved the efficiency of drugs reaching the lesion, bringing new opportunities for tumor treatment. Therefore, designing and preparing novel nano-drug delivery systems has gradually become one of the effective ways to treat tumors.

[0003] In a series of new nano-drug delivery systems, functional hybrid nanomaterials combine the charact...

Examples

Embodiment 1

[0035] Example 1 Preparation method of gold nanoparticle vesicles for controlled drug release by ultraviolet light

[0036] This preparation method mainly comprises the following steps:

[0037] 1. Synthesis of photoresponsive polymer: It is worth noting that the photoresponsive group in the photoresponsive polymer is exemplified by coca isodioic acid, which can The four-membered ring will be broken, so that the hydrophilic and hydrophobic segments will be separated, and the chain structure of the polymer will be changed, so as to achieve the purpose of light-controlled drug release.

[0038] 1.1. Synthesis of the photoresponsive group: cinnamic acid was ring-closed under 365nm ultraviolet light irradiation to form the ultraviolet photoresponsive group coca-metadioic acid.

[0039] 1.2. Synthesis of polymers containing (S-S) bonds and coca ishalic acid:

[0040] 1.2.1. Synthesis of polystyrene (PS-coca-isodiacid) capped with coca-isodiacid:

[0041] (a) Monohydroxyl-termina...

Embodiment 6

[0068] Example 6 Disassembly test of gold nanoparticle vesicles releasing drugs in response to ultraviolet light

[0069] The gold nanoparticle vesicles that release drugs in response to ultraviolet light described in Example 1 were dispersed in water, and dynamic light scattering (DLS) was used to detect the disassembly process of the gold nanoparticle vesicles in real time. The particle size changes of the gold nanoparticle vesicles were monitored after 0min, 10min, 20min, and 30min of ultraviolet radiation, namely image 3 . The results showed that the vesicle size decreased successively after each UV irradiation, so the vesicle disassembly process could be further controlled by adjusting the UV irradiation time.

Embodiment 7

[0070] Example 7 Photothermal effect test of nanoparticle vesicles releasing drugs in response to ultraviolet light

[0071] The gold nanoparticle vesicles that release drugs in response to ultraviolet light described in Example 1 were dispersed in water, and their photothermal effects were measured at 0 μg / mL, 25 μg / mL, 50 μg / mL, 75 μg / mL, and 100 μg / mL ,Right now Figure 4 . The results showed that the photothermal effect of the gold nanoparticle vesicles was getting better and better with the increase of the concentration, which indicated that the gold nanoparticle vesicles had a better photothermal effect.

technical field

[0001] The invention belongs to the field of drug controlled release, and more specifically relates to a light-controlled nanoparticle assembly and its preparation method and application. Background technique

[0002] Cancer (ie, malignant tumor) is one of the major diseases that endanger human health and life. Chemotherapy (i.e. chemotherapy), as one of the cancer treatment methods, has obstacles and toxic side effects to anticancer drugs due to the physical environment of the body, thus limiting the effect of chemotherapy to a large extent. In recent years, the rapid development of nano-drug delivery systems has greatly improved the efficiency of drugs reaching the lesion, bringing new opportunities for tumor treatment. Therefore, designing and preparing novel nano-drug delivery systems has gradually become one of the effective ways to treat tumors.

[0003] In a series of new nano-drug delivery systems, functional hybrid nanomaterials combine the charact...

Examples

Embodiment 1

[0035] Example 1 Preparation method of gold nanoparticle vesicles for controlled drug release by ultraviolet light

[0036] This preparation method mainly comprises the following steps:

[0037] 1. Synthesis of photoresponsive polymer: It is worth noting that the photoresponsive group in the photoresponsive polymer is exemplified by coca isodioic acid, which can The four-membered ring will be broken, so that the hydrophilic and hydrophobic segments will be separated, and the chain structure of the polymer will be changed, so as to achieve the purpose of light-controlled drug release.

[0038] 1.1. Synthesis of the photoresponsive group: cinnamic acid was ring-closed under 365nm ultraviolet light irradiation to form the ultraviolet photoresponsive group coca-metadioic acid.

[0039] 1.2. Synthesis of polymers containing (S-S) bonds and coca ishalic acid:

[0040] 1.2.1. Synthesis of polystyrene (PS-coca-isodiacid) capped with coca-isodiacid:

[0041] (a) Monohydroxyl-termina...

Embodiment 6

[0068] Example 6 Disassembly test of gold nanoparticle vesicles releasing drugs in response to ultraviolet light

[0069] The gold nanoparticle vesicles that release drugs in response to ultraviolet light described in Example 1 were dispersed in water, and dynamic light scattering (DLS) was used to detect the disassembly process of the gold nanoparticle vesicles in real time. The particle size changes of the gold nanoparticle vesicles were monitored after 0min, 10min, 20min, and 30min of ultraviolet radiation, namely image 3 . The results showed that the vesicle size decreased successively after each UV irradiation, so the vesicle disassembly process could be further controlled by adjusting the UV irradiation time.

Embodiment 7

[0070] Example 7 Photothermal effect test of nanoparticle vesicles releasing drugs in response to ultraviolet light

[0071] The gold nanoparticle vesicles that release drugs in response to ultraviolet light described in Example 1 were dispersed in water, and their photothermal effects were measured at 0 μg / mL, 25 μg / mL, 50 μg / mL, 75 μg / mL, and 100 μg / mL ,Right now Figure 4 . The results showed that the photothermal effect of the gold nanoparticle vesicles was getting better and better with the increase of the concentration, which indicated that the gold nanoparticle vesicles had a better photothermal effect.

Images