Preparation method for mixed culture fermented compound eucommia ulmoides leaf powder

A technology of Eucommia eucommia leaf powder and compound strains, which is applied in the directions of medical preparations, antibacterial drugs, and drug combinations containing active ingredients, which can solve the problem that eucommia gum is difficult to dissolve, affects the efficacy of compound eucommia leaf powder, and the dissolution rate of eucommia gum. Low problems, to achieve excellent effect of disease prevention and growth promotion

Inactive Publication Date: 2019-03-22
四川爱邦伟业生物工程有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Compound eucommia leaf powder is an animal feed additive developed by the applicant. It is composed of astragalus, codonopsis, sealwort, ganoderma, honeysuckle, isatidis, and eucommia leaves. Astragalus, the main component of Astragalus polysaccharides are embedded in cellulose, some of the astragalus polysaccharides and fibers form a bonded state, it is extremely difficult to dissolve, Eucommia contains a large amount of Eucommia gum, Eucommia gum is difficult to dissolve, the processing of ordinary Chinese medicine is to fry the bark of Eucommia ulmoides Part of the eucommia gum is degraded, but the dissolution rate of the eucommia gum is still very low, which seriously affects the efficacy of the compound eucommia leaf powder

Method used

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  • Preparation method for mixed culture fermented compound eucommia ulmoides leaf powder
  • Preparation method for mixed culture fermented compound eucommia ulmoides leaf powder
  • Preparation method for mixed culture fermented compound eucommia ulmoides leaf powder

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preparation example Construction

[0035] The steps of the preparation method of compound bacterial strain fermentation compound Eucommia leaf powder of the present invention are as follows:

[0036] (1) Preparation of compound strains: put the original bacterial solutions of Bacillus subtilis, Saccharomyces cerevisiae, and interrhizobium astragalus into different triangular flasks from the inclined plane, and carry out the expansion culture by shaking culture for 48 hours. After the expansion culture, each bacteria The total number of colonies of species is: the total number of colonies of Saccharomyces cerevisiae is 3.8×10 3 cfu / g, the total number of colonies of Bacillus subtilis is 2.6×10 3 cfu / g, the total number of colonies of Astragalus interrhizobia was 2.8×10 3 cfu / g; mixed according to the following volume percentages: 5-90% of Saccharomyces cerevisiae, 5-90% of Bacillus subtilis, and 5-90% of interrhizobia astragalus to obtain composite strains;

[0037] (2) Preparation of fermented traditional Chi...

Embodiment 1

[0042] Each composition in the compound eucommia leaf powder is calculated by weight: 50 parts of astragalus, 50 parts of codonopsis, 50 parts of sealwort, 50 parts of honeysuckle, 50 parts of eucommia leaves, 50 parts of isatidis, and 50 parts of ganoderma lucidum.

[0043] The steps of the preparation method of compound bacterial strain fermentation compound Eucommia leaf powder of the present invention are as follows:

[0044] (1) Preparation of compound strains: put the original bacterial solutions of Bacillus subtilis, Saccharomyces cerevisiae, and interrhizobium astragalus into different triangular flasks from the inclined plane, and shake culture for 48 hours to expand the culture. The total number of colonies of each strain The total number of colonies for Saccharomyces cerevisiae was 3.8 x 103 cfu / g, the total number of colonies of Bacillus subtilis is 2.6×10 3 cfu / g, the total number of colonies of Astragalus interrhizobia was 2.8×10 3 cfu / g; mixed according to the ...

Embodiment 2

[0049] Each composition in the compound eucommia leaf powder is calculated by weight: 30 parts of astragalus, 40 parts of codonopsis, 20 parts of sealwort, 50 parts of honeysuckle, 50 parts of eucommia leaves, 30 parts of isatidis, and 20 parts of ganoderma lucidum.

[0050] The steps of the preparation method of compound bacterial strain fermentation compound Eucommia leaf powder of the present invention are as follows:

[0051] (1) Preparation of compound strains: put the original bacterial solutions of Bacillus subtilis, Saccharomyces cerevisiae, and interrhizobium astragalus into different triangular flasks from the inclined plane, and shake culture for 48 hours to expand the culture. The total number of colonies of each strain For: The total number of colonies of Saccharomyces cerevisiae is 3.8×10 3 cfu / g, the total number of colonies of Bacillus subtilis is 2.6×10 3 cfu / g, the total number of colonies of Astragalus interrhizobia was 2.8×10 3 cfu / g; mixed according to t...

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Abstract

The invention provides a preparation method for mixed culture fermented compound eucommia ulmoides leaf powder. The preparation method comprises the following steps that (1) a mixed culture is prepared, specifically, bacillus subtilis, saccharomyces cerevisiae and astragalus membranaceus intermediate rhizobium are propagated and mixed so as to obtain the mixed culture; (2) a fermented traditionalChinese medicine culture medium is prepared, specifically, the culture medium containing a compound eucommia ulmoides leaf powder body is prepared; and (3) fermentation is carried out, specifically, the fermented traditional Chinese medicine culture medium is inoculated with the mixed culture, aerobic fermentation is carried out, and when the pH value is close to 5, the fermentation end point is reached. The invention further provides the corresponding fermented compound eucommia ulmoides leaf powder. The fermented compound eucommia ulmoides leaf powder is traditional Chinese medicine probiotics which is free of drug residues and excellent in disease prevention and growth promotion effects; and due to the synergistic effect of mixed bacteria and traditional Chinese medicines, an additive produced by using the mixed culture fermented compound eucommia ulmoides leaf powder is far superior to other similar products in application effect.

Description

technical field [0001] The invention relates to a fermentation method for adding traditional Chinese medicine in the process of microorganism propagation, in particular to a preparation method for fermenting compound eucommia leaf powder by using compound strains. Background technique [0002] Compound eucommia leaf powder is an animal feed additive developed by the applicant, which is composed of astragalus, codonopsis, sealwort, ganoderma, honeysuckle, isatidis, and eucommia leaves. Astragalus, the main component of Astragalus polysaccharides is embedded in cellulose, some of the astragalus polysaccharides and fibers form a bonded state, which is extremely difficult to dissolve. Eucommia contains a large amount of Eucommia gum, which is difficult to dissolve. Common Chinese medicine is prepared by frying Eucommia bark However, the dissolution rate of Eucommia gum is still very low, which seriously affects the efficacy of compound Eucommia leaf powder. [0003] Traditional...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8969A61P31/04A61P31/12A61P37/04A23K10/30A23K10/12A23K10/18A23K10/37A23K20/105A23K20/22A61K35/742A61K35/74A61K36/064
CPCA61K35/74A61K35/742A61K36/064A61K36/074A61K36/195A61K36/315A61K36/344A61K36/355A61K36/46A61K36/481A61K36/8969A61K2236/19A23K10/12A23K10/18A23K10/30A23K10/37A23K20/105A23K20/22A61P31/04A61P31/12A61P37/04A61K2300/00Y02P60/87
Inventor 雷春林余智勇张伟
Owner 四川爱邦伟业生物工程有限责任公司
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