Sturgeon source Streptococcus dysgalactiae and application thereof

A technology derived from Streptococcus dysgalactiae and Sturgeon, which is applied in the direction of bacteria, antibacterial drugs, and veterinary vaccines. Strong resistance, good immune protection, and simple technical process

Active Publication Date: 2019-03-26
ZHEJIANG INST OF FRESH WATER FISHERIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Streptococcus iniae and Streptococcus agalactiae have two serotypes, and the virulence and pathogenicity of different serotypes are quite different, while Streptococcus dysgalactiae has not been found to have different serotypes

Method used

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  • Sturgeon source Streptococcus dysgalactiae and application thereof
  • Sturgeon source Streptococcus dysgalactiae and application thereof
  • Sturgeon source Streptococcus dysgalactiae and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Isolation, identification and toxicity test of Streptococcus dysgalactiae SDS strain from Sturgeon

[0024] 1. Isolation of Streptococcus dysgalactiae from Sturgeon:

[0025] In 2017, disease materials were collected from a Streptococcus-infected fish farm in Quzhou, Zhejiang Province. Kidney and brain tissues of the affected sturgeon were aseptically collected, inoculated on THA agar medium, cultured at 28-30°C for 24-36 hours, and then a typical single colony was selected. Carry out purification culture. The purified single colony was selected and inoculated in THB liquid culture medium, and cultivated overnight at 30° C. on a shaker (220 r / min). The pure culture was inoculated on an agar plate containing sheep blood, and after culturing at 30°C for 24 hours, transparent and round colonies with a diameter of 1 to 2 mm could be seen to form α-hemolytic ( figure 1 ). Pick the above-mentioned single colonies for Gram staining, and observe under the microscop...

Embodiment 2

[0038]Example 2 Preparation of Streptococcus dysgalactiae Vaccine from Sturgeon and Detection of Immune Efficacy

[0039] 1. Preparation of Streptococcus dysgalactiae vaccine from Sturgeon

[0040] Vaccines are prepared by:

[0041] 1) Preparation of first-level seed liquid: Inoculate the SDS strain of Streptococcus dysgalactiae from Streptococcus galactiae from Streptococcus galactiae from freeze-dried preservation on a THA solid plate, and culture it at 30°C for 18 hours; then pick a single colony in THB medium (containing 5 % newborn bovine serum), at a temperature of 30°C, shake at a speed of 120rpm for 18h, and after passing the pure test, use it as a first-class seed solution and store it at 2-8°C for later use, and the storage time does not exceed 3 days. .

[0042] 2) Preparation of secondary seed liquid: take the primary seed liquid obtained in step 1), inoculate it in THB medium according to the volume ratio of seed liquid to medium of 1:20, and inoculate at a temp...

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Abstract

The invention discloses sturgeon source Streptococcus dysgalactiae and application thereof. The sturgeon source Streptococcus dysgalactiae is preserved at the China typical culture preservation centerof the Wuhan University (on a campus of the Wuhan University, No.299, Bayi Road, Wuchang District, Wuhan City, Hubei Province) in November 29th, 2018, the preservation number is CCTCC M 2018843, andthe classified name of a bacterial strain is Streptococcus dysgalactiae SDS. The invention further discloses the application of the sturgeon source Streptococcus dysgalactiae to a drug for preventionand control over sturgeon streptococcicosis. The drug can serve as a vaccine, the vaccine has good immune protection capability, sturgeon is subjected to immunization through the vaccine and thus canresist disoperation of streptococcosis of domestic main sturgeon breeding varieties, and market prospects are broad.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to Streptococcus dysgalactiae from Sturgeon and its application. Background technique [0002] The main species of sturgeon farming in China are Siberian sturgeon (Acipenser baerii), Schrencki (Acipenser schrencki) and so on. Among them, the Siberian sturgeon has become the leading species of domestic sturgeon farming due to its easy domestication, fast growth, large body and good meat quality. With the development of Chinese sturgeon farming, infectious diseases are becoming more and more serious. There have been many reports on the pathogenicity of Aeromonas hydrophila (Aeromonas hydrophila) infection of various sturgeons, and about the infection of sturgeons by Aeromonas caviae (Aeromonas carvia) and Plesimonas shigelloides Reports of disease in fish. The pathogenic bacteria reported above are all Gram-negative bacteria, only Roy and Ruth et ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/09A61P31/04C12R1/46
CPCA61K39/092A61K2039/521A61K2039/552A61P31/04C12N1/205C12R2001/46
Inventor 沈锦玉姚嘉赟潘晓艺蔺凌云徐洋尹文林曹铮许式见胡谋邵辉
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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