Method for improving performance of fish flesh myosin
A technology of myosin and fish meat, which is applied in the field of improving the thermal stability and solubility of fish myosin, improving the performance of fish myosin, and can solve the problems of lack of explanation, so as to reduce hydrophobic interaction and improve thermal stability , Inhibit the effect of thermal denaturation aggregation
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Embodiment 1
[0020] A method for improving fish myosin performance, comprising the following steps:
[0021] S1. Slaughter fresh fish, take its white meat part, extract myosin, and obtain myosin stock solution;
[0022] S2. Put the myosin stock solution into a dialysis bag, put it into a treatment solution at pH 7.0 for dialysis, the treatment solution includes 150 mmol / L salt solution, 5 mmol / L amino acid solution, and dialyze for 1 hour;
[0023] S3. Add molecular chaperone solution to the treatment solution, and make the final concentration at 70mmol / L;
[0024] S4. Continue dialysis for 11 hours;
[0025] S5. Pour out the dialyzed myosin stock solution, and cool down to room temperature naturally.
[0026] Further, the amino acid solution includes the following components in parts by weight: 5 parts of lysine, 6 parts of histidine, 3 parts of glycine, 8 parts of arginine, and 3 parts of glycerol.
[0027] Further, the molecular chaperone solution includes the following components in...
Embodiment 2
[0032]A method for improving fish myosin performance, comprising the following steps:
[0033] S1. Slaughter fresh fish, take its white meat part, extract myosin, and obtain myosin stock solution;
[0034] S2. Put the myosin stock solution into a dialysis bag, put it into a treatment solution at pH 10.0 for dialysis, the treatment solution includes 400 mmol / L salt solution, 25 mmol / L amino acid solution, and dialyze for 3 hours;
[0035] S3. Add molecular chaperone solution to the treatment solution, and make the final concentration at 190 mmol / L;
[0036] S4. Continue dialysis for 18 hours;
[0037] S5. Pour out the dialyzed myosin stock solution, and cool down to room temperature naturally.
[0038] Further, the amino acid solution includes the following components in parts by weight: 18 lysine, 14 histidine, 10 glycine, 19 arginine, 7 glycerol.
[0039] Further, the molecular chaperone solution includes the following components in parts by weight: 13 parts by weight of p...
Embodiment 3
[0044] A method for improving fish myosin performance, comprising the following steps:
[0045] S1. Slaughter fresh fish, take its white meat part, extract myosin, and obtain myosin stock solution;
[0046] S2. Put the myosin stock solution into a dialysis bag, put it into the treatment solution with pH 7.8 for dialysis, the treatment solution includes 220 mmol / L salt solution, 12 mmol / L amino acid solution, and dialyze for 1.6h;
[0047] S3. Add molecular chaperone solution to the treatment solution, and make the final concentration at 110 mmol / L;
[0048] S4. Continue dialysis for 13 hours;
[0049] S5. Pour out the dialyzed myosin stock solution, and cool down to room temperature naturally.
[0050] Further, the amino acid solution includes the following components in parts by weight: 8 parts of lysine, 9 parts of histidine, 6 parts of glycine, 10 parts of arginine, and 4 parts of glycerol.
[0051] Further, the molecular chaperone liquid includes the following component...
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