Application of carbonic anhydrase-2 as a detection marker in the diagnosis of kidney stones

A carbonic anhydrase, detection label technology, applied in the field of biomedical diagnosis

Active Publication Date: 2021-09-10
九江学院附属医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, many patients with kidney stones after treatment maintain a persistently high level of urinary carbonic anhydrase-2, and the risk of recurrence is much greater than those patients whose urine carbonic anhydrase-2 returns to normal levels after treatment for kidney stones

Method used

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  • Application of carbonic anhydrase-2 as a detection marker in the diagnosis of kidney stones
  • Application of carbonic anhydrase-2 as a detection marker in the diagnosis of kidney stones
  • Application of carbonic anhydrase-2 as a detection marker in the diagnosis of kidney stones

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Select 3 patients with kidney stones and 3 normal patients, and collect the urine of the above patients for the screening of proteomics technology. The above-mentioned people are all male, and their age and weight are roughly similar.

[0037] The inventor screened 8 proteinuria markers in the early stage, of which only carbonic anhydrase-2 was stably expressed in the urine of patients with kidney stones.

[0038] Inclusion criteria of the test population: the inventor included healthy people, patients with kidney stones and patients with kidney tumors from January 2017 to October 2018. The following implementation steps can only be carried out after the above-mentioned population meets the following criteria:

[0039] (1) Age 18-68;

[0040] (2) Urinary tract color Doppler ultrasound, X-ray and CT examinations were performed to confirm the diagnosis of patients with kidney stones;

[0041] (3) All patients with renal tumors underwent surgery and were diagnosed throug...

Embodiment 2

[0056] The detection method of carbonic anhydrase-2 content and activity, comprises the following steps:

[0057] (1) Collect the early morning urine of the subject and send it for inspection within 2 hours. If it cannot be sent for inspection in time, it should be stored at 5°C, but the storage time is required to be less than 24 hours. The urine needs to be centrifuged at 1800r / min before testing. After 10 minutes, take supernatant samples for testing;

[0058] (2) Take out the microwell ELISA plate, set standard wells and sample wells, add 50 μL of carbonic anhydrase-2 standard substances of different concentrations to each of the standard wells, and add 50 μL of the sample to be tested in the sample wells;

[0059] (3) Add 100 μL of horseradish peroxidase-labeled carbonic anhydrase-2 antibody to each well of standard wells and sample wells, and incubate in a 37°C water bath for 65 minutes;

[0060] (4) Discard the liquid, pat dry on absorbent paper, fill each well with wa...

Embodiment 3

[0065] The detection method of carbonic anhydrase-2 content and activity, comprises the following steps:

[0066] (1) Collect the early morning urine of the subject and send it for inspection within 2 hours. If it cannot be sent for inspection in time, it should be stored at 6°C, but the storage time is required to be less than 24 hours. The urine needs to be centrifuged at 2000r / min before testing. 6min, take the supernatant sample to be detected;

[0067] (2) Take out the microwell ELISA plate, set standard wells and sample wells, add 50 μL of carbonic anhydrase-2 standard substances of different concentrations to each of the standard wells, and add 50 μL of the sample to be tested in the sample wells;

[0068] (3) Add 100 μL of horseradish peroxidase-labeled carbonic anhydrase-2 antibody to each well of standard wells and sample wells, and incubate in a 37°C water bath for 65 minutes;

[0069] (4) Discard the liquid, pat dry on absorbent paper, fill each well with washing ...

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Abstract

The present invention provides the application of carbonic anhydrase-2 as a detection marker in the diagnosis of kidney stones, and relates to the field of biomedical diagnosis, specifically the application of carbonic anhydrase-2 as a detection marker in the diagnosis of kidney stones. By detecting carbonic anhydrase ‑2 content and activity to diagnose kidney stones, carbonic anhydrase ‑2 includes multiple species and subtypes, has a high degree of homology, and detection of different species and subtypes can often achieve the same effect. The present invention has verified that urine Liquid carbonic anhydrase-2 was significantly elevated in patients with kidney stones, which can clearly distinguish healthy people from patients with renal tumors. At the same time, the diagnostic value of urine carbonic anhydrase-2 in the detection of kidney stone patients was further verified. It has the advantages of high diagnostic sensitivity and specificity, and convenient detection. , It is convenient for clinicians to give corresponding prevention and treatment measures, which reduces the economic burden and physical pain of patients.

Description

technical field [0001] The invention relates to the field of biomedical diagnosis, in particular to the application of carbonic anhydrase-2 as a detection marker in the diagnosis of kidney stones. Background technique [0002] Kidney calculus is a common disease of the urinary system, frequently-occurring, more males than females, mostly in young adults, and there is no significant difference in the incidence rate between the left and right sides. 40% to 75% of patients with kidney stones have different degrees of low back pain. Larger stones with little movement, manifested as soreness and discomfort in the waist, or dull or dull pain when physical activity increases. Colic pain caused by small stones often occurs suddenly with severe pain in the waist and abdomen, which is paroxysmal. [0003] The main method of judging whether a patient has kidney stones is to rely on imaging methods such as color Doppler ultrasound, X-ray and CT. Although imaging methods are widely us...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/573G01N33/535
CPCG01N33/535G01N33/573G01N2333/988G01N2800/345
Inventor 冷远景
Owner 九江学院附属医院
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