Use of extracts of deschampsia antarctica for counteracting human skin barrier damage caused by environmental aggressions
A skin barrier, extract technology, applied in the field of cosmetics or dermatology, can solve the problems of aggravating the ability of skin protectants, not disclosing EDA, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0075] Example 1 - Protective effect of pretreatment with EDA against dehydration and hyperosmotic shock
[0076] The aim of this experiment was to evaluate the possible protective effect of pretreatment in human fibroblasts with Antarctic hair grass extract against dehydration or hyperosmolar shock due to lack of air humidity, measuring changes in cell survival.
[0077] Materials and methods:
[0078] - Products to be tested:
[0079] Antarctic hair grass extract (EDA): In experiments with fibroblasts, EDA dried with maltodextrin, batch 160215, was used. Dissolve the product at a concentration of 10 mg / ml (equivalent to 2.1 mg of EDA / ml) in Duchenne's Modified Eagle Medium (DMEM) medium, stir in a magnetic stirrer for 30 min, and filter through 0.2 μm sterile cellulose acetate filter (Minisart, Sartorius).
[0080] - Media and reagents:
[0081] Phenol red-free DMEM medium (Dulman's modified Eagle medium) (Sigma), DMEM medium (Sigma), penicillin-streptomycin (Sigma),...
Embodiment 2
[0105] Example 2 - Protective effect of EDA pretreatment on environmental heat stress
[0106] The purpose of this experiment was to evaluate the possible protective effect of pretreatment of Antarctic hair grass extract in the immortalized human keratinocyte line HaCaT on heat stress (cold or heat), first with regard to the survival rate, and secondly with regard to its The effect was evaluated as the effect on mitochondrial potential.
[0107] Materials and methods:
[0108] - Products to be tested:
[0109]Freeze-dried Antarctic hair grass extract (EDA), lot 13E04. The product was directly dissolved in standard buffer (see below) at a concentration of 1 mg / ml, and 100 μl of this solution was used.
[0110] Starch dried Antarctic hair grass extract (EDA), lot 200314. EDA content: 0.25g EDA / g product. The product was suspended in standard buffer at a concentration of 10 mg / ml (equivalent to 2.5 mg of EDA / ml), stirred on a magnetic stirrer for 30 min, and passed throu...
Embodiment 3
[0159] Embodiment 3-protective effect on environmental pollution (tobacco smoke) with EDA pretreatment
[0160] The purpose of this experiment was to evaluate the possible protective effect of pretreatment of Antarctic hair grass extract in human fibroblasts or transformed keratinocytes against environmental pollution such as environmental pollution caused by tobacco smoke, the effect was evaluated with respect to cell survival .
[0161] Materials and methods:
[0162] - Products to be tested:
[0163] Antarctic hair grass extract (EDA): In experiments with fibroblasts, EDA dried with starch, batch 150414 was used. EDA content: 0.25g EDA / g product. The product was suspended in complete DMEM medium at a concentration of 10 mg / ml (equivalent to 2.5 mg of EDA / ml), stirred in a magnetic stirrer for 30 minutes, and passed through a 0.2 μm sterile cellulose acetate filter (Minisart, Sartorius )filter. In experiments with keratinocytes, EDA adsorbed on maltodextrin, lot 16021...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More