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Endometriosis fibrosis animal model construction method and application thereof

A technology of endometrium and animal models, applied in the direction of drug combinations, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve the problems of unreported animal models of endometriosis fibrosis, and achieve needle insertion Easy, clear genetic background, convenient feeding effect

Inactive Publication Date: 2019-04-05
SHANGHAI CHANGHAI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But about a kind of endometriosis fibrosis animal model construction method of the present invention and its application, there is no report yet

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  • Endometriosis fibrosis animal model construction method and application thereof
  • Endometriosis fibrosis animal model construction method and application thereof
  • Endometriosis fibrosis animal model construction method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0041] The establishment of embodiment 1 endometriosis fibrosis animal model

[0042] In this example, the endometriosis fibrosis animal model was constructed by subcutaneously injecting estrogen into the neck and back of 7-week-old sexually mature BALB / c female mice combined with intraperitoneal injection of endometrial tissue fragments. fibrosis model.

[0043] 1 experimental animal

[0044] Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. purchased 42 7-week-old sexually mature BALB / c female mice, weighing 18-20g.

[0045] 2 Experimental methods

[0046] The animals used in the present invention are 7-week-old sexually mature BALB / c female mice with a body weight of 18-20 g. A total of 42 BALB / c mice were purchased, 36 of which were used for model establishment, and the other 6 were used as controls (intraperitoneal injection of adipose tissue) for model comparison and identification. The 36 mice used for model establishment were randomly divided into 12 d...

Embodiment 2

[0068] 1 experimental animal

[0069] Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. purchased 36 7-week-old sexually mature BALB / c female mice, weighing 18-20g; 24 female mature unmated SD rats, weighing 180-220g, all in Raised under SPF environment.

[0070] 2 Experimental methods

[0071] 2.1 Grouping

[0072] Divided into experimental group and control group, the experimental group selected 36 7-week-old sexually mature BALB / c female mice weighing 18-20g as experimental animals; the control group selected 24 mature female unmated SD mice weighing 180-220g Rats were used as experimental animals.

[0073] 2.2 Specific methods

[0074] 2.2.1 The mice in the experimental group were given the following methods:

[0075] (1) Preparation of estrogen solution: dissolve estradiol benzoate powder with sesame oil for experimentation, make a working solution with a concentration of 30 μg / ml and store it in a 4-degree refrigerator in the dark for future use;

[00...

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Abstract

The invention relates to an endometriosis fibrosis animal model construction method and application thereof and belongs to the technical field of animal models. According to a preparation method of anendometriosis fibrosis animal model, estrogen is subcutaneously injected to neck and back of an SPF-grade BALB / c female mouse and endometrial tissue fragments are intraperitoneally injected, so thatthe endometriosis fibrosis animal model is constructed. The preparation method has the advantages that the model has high conformity with clinical pathologic characteristics of endometriosis; the model is convenient to operate, the modeling period is short and the modeling success rate is high; an efficient modeling method is provided for researches of the endometriosis. Meanwhile, the model can be used as an ideal model for exploring the pathogenesis and an effective diagnosis and treatment strategy of endometriosis fibrosis.

Description

technical field [0001] The invention relates to a method for constructing an endometriosis fibrosis animal model and its application. Background technique [0002] Endometriosis is an estrogen-dependent disease in which endometrial glands and stroma are planted outside the uterus. The incidence rate of women of childbearing age is 10%-15%. It causes severe dysmenorrhea, dyspareunia, infertility, etc. Affect women's physical and mental health and quality of life. Due to the wide incidence of the disease, various symptoms, and malignant behaviors of metastasis and recurrence, its etiology and pathogenesis are still unclear. The current treatment methods mainly include surgery and hormone drugs, but the curative effect is not satisfactory. Therefore, endometriosis is the focus and difficulty of current research. [0003] The establishment of animal models of endometriosis is helpful for the understanding of the disease and the development of new drugs. At present, apart from...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/565A61P15/00
CPCA61K31/565A61P15/00
Inventor 俞超芹毕艳丽倪喆鑫李溪
Owner SHANGHAI CHANGHAI HOSPITAL
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