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Separation and preparation of dihydrosanguinarine and application thereof in preparation of anti-inflammatory drugs

A technology of dihydrosanguinarine and anti-inflammatory drugs, which is applied in the field of medicine and can solve problems such as the lack of dihydrosanguinarine.

Inactive Publication Date: 2019-04-05
JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the application of dihydrosanguinarine in anti-inflammation

Method used

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  • Separation and preparation of dihydrosanguinarine and application thereof in preparation of anti-inflammatory drugs
  • Separation and preparation of dihydrosanguinarine and application thereof in preparation of anti-inflammatory drugs
  • Separation and preparation of dihydrosanguinarine and application thereof in preparation of anti-inflammatory drugs

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Embodiment

[0029] Example: Dihydrosanguinarine has a good down-regulation effect on LPS-induced inflammation in RAW 264.7 cells.

[0030] (1) Separation and preparation of dihydrosanginarine

[0031] Take Kudiding medicinal material, add 80% ethanol aqueous solution according to the material-liquid ratio of 1:10, condense and reflux and extract twice, each time for 1.5h, to obtain the alcohol extract. It was concentrated under reduced pressure to obtain an extract, which was redissolved with 2% hydrochloric acid and extracted 4 to 5 times with petroleum ether to obtain a petroleum ether layer extract. The pH of the aqueous solution was adjusted to 10 with ammonia water, and extracted 6-7 times with dichloromethane to obtain a dichloromethane layer extract. Extracts from the petroleum ether layer and the dichloromethane layer were concentrated under reduced pressure to finally obtain the extract. Through the traditional silica gel column chromatography separation technology with petrole...

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Abstract

The invention belongs to the technical field of medicine and particularly relates to a separation and preparation method of dihydrosanguinarine and application thereof in the preparation of anti-inflammatory drugs. The dihydrosanguinarine (to LC-PDA analytical purity, under structural identification via MS, 1H-NMR and 13C-NMR) is attained by performing condensing reflux extraction on bunge corydalis herb, concentrating, extracting with an organic solvent, and performing column chromatography separation. The dihydrosanguinarine can evidently inhibit transcription of LPS-induced RAW 264.7 cell inflammatory gene iNOS, TNF-alpha, IL-1 beta and IL-6, thereby inhibiting the release of inflammatory medium NO and the expression level of TNF-alpha, IL-1 beta and IL-6 proteins. The research on anti-inflammatory effect mechanism shows that the dihydrosanguinarine can inhibit the expression level of TLR4 and TLR2 in LPS-induced RAW 264.7 cell inflammatory signal pathways and the phosphorylation ofI kappa B alpha and p65 in NF-kappa B, namely the dihydrosanguinarine can resist inflammation by inhibiting the activation of TLRs / NF-kappa B inflammation signal pathway.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the separation and preparation of dihydrosanguinarine from the traditional Chinese medicine Kudiding and its application in the preparation of anti-inflammatory drugs. Background technique [0002] Kudiding, also known as Xiaojicai, is the dried rooted whole plant of Corydalisbungeana Turcz (Papaveraceae) and is included in the 2015 edition of the Chinese Pharmacopoeia and the "Ministry of Health Drug Standard Mongolian Medicine". Its production areas are distributed in Liaoning, Hebei, Shandong, Shanxi and other places. Its nature and flavor are pungent, bitter, and cold. It can clear away heat and detoxify, dissipate stagnation and reduce swelling. It has been reported in the literature that Kudidin mainly contains alkaloid components, and dihydrosanguinarine is one of the main alkaloid components isolated from Kudidin (Huang Ge, Li Famei. Separation and structural...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/153A61P29/00
CPCC07D491/153
Inventor 朱粉霞张浩李木子
Owner JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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