Coliform detection device and detection method thereof

Abstract

The invention relates to the technical field of microbiological detection, in particular to a coliform detection device and a detection method thereof. The coliform detection device comprises a watertaking bag, a water taking bag connector assembly and a detection bag; and the water taking bag connector assembly comprises a guide pipe and further comprises a water taking bag connector and a Luermale connector which are arranged at the two ends of the guide pipe separately. According to the coliform detection device and the detection method thereof, during detection, the water taking bag connector is hermetically connected to a silica gel valve at a water taking bag port in a matched mode, the Luer male connector is hermetically connected to a detection bag one-way valve on the detectionbag, a water sample is transferred from the water taking bag to the detection bag in a fully-closed state to be mixed with a medium, then culture is carried out, and coliform detection is carried out,and therefore the operation is simple, the pollution possibly caused in the detection process is avoided, and an accurate detection result is obtained; and a MMO-MUG dehydrated medium, an LECC dehydrated medium or a lactose peptone dehydrated medium serves as a detection medium, and coliform can be accurately detected by carrying out culture for 18-28 hours.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, and in particular relates to a coliform detection device and a detection method thereof. Background technique [0002] In a microgravity environment, the pathogenicity of coliform bacteria is enhanced and it is more likely to cause disease (Chin JAerospace Med, June 2010, Vol.21, No.2, p144-147), so timely detection of coliform bacteria and prevention are very important. [0003] In 2016, NASA used a hand-held mini-sequencer to collect and culture specimens, and it took at least one week of testing time to detect coliform bacteria on the samples to be tested. However, this method is complex and time-consuming. [0004] The commonly used detection methods for coliform bacteria include enzyme substrate method and multi-tube fermentation method. The detection principle of enzyme substrate method is that coliform bacteria grow in the chromogenic medium to produce enzymes, decompose t...

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Problems solved by technology

[0006] Therefore, the technical problem to be solved by the present invention is to overcome the technical problems of complex coliform detection operations in the microgravity non-clean state and the technical problems of easily polluted results resulting in inaccurate results in the prior art, and to provide a microgravity non-clean state. The coliform detection device and detection method in which the sample is transferred to the detection bag in a fully enclosed state and mixed with the culture medium in the environment, making the operation simple and fast, avoiding contamination and obtaining accurate detection results

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