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Protein chip for detecting residues of veterinary drugs in milk and preparation method of protein chip

A protein chip and veterinary drug residue technology, applied in the biological field, can solve the problems of inability to be adopted on a large scale, high detection cost, and slow detection speed, and achieve the effects of simplifying the pre-processing process, high accuracy of results, and reducing detection costs

Inactive Publication Date: 2019-04-12
江苏三联生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these six indicators can be measured, there are disadvantages such as slow detection speed and high detection cost.
Moreover, there are practical problems such as inconvenient operation and high error probability in the separate detection of the six indicators.
As the most authoritative HPLC (liquid chromatography) method for trace component detection, although the result is accurate and reliable, the cost is extremely high, the equipment is expensive, and it cannot be adopted on a large scale.
Whether milk contains antibiotics and whether it is safe to eat is a question that the public is eager to know. In order to speed up the detection, many companies, testing agencies, and government law enforcement agencies have to spend more manpower, material resources, and time for testing. MA, CAP, PCN, SAS, GM, TC, causing a lot of waste

Method used

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  • Protein chip for detecting residues of veterinary drugs in milk and preparation method of protein chip
  • Protein chip for detecting residues of veterinary drugs in milk and preparation method of protein chip
  • Protein chip for detecting residues of veterinary drugs in milk and preparation method of protein chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A protein chip for the detection of veterinary drug residues, the preparation method of the protein chip comprises the following steps:

[0036] (1) Black slide pretreatment;

[0037] ① Soak the black slide in the slide pretreatment solution containing 2% NaOH for 16 hours, and then wash it with purified water for 2 to 8 times;

[0038] ② Soak black slides in 0.05% silane solution (medium: 25% ethanol) for 60 minutes;

[0039] ③Purge the soaked black glass slides with nitrogen gas, put them in an oven, and bake them at 180°C for 0.2h.

[0040] (2) Antigen solution spotting;

[0041] refer to figure 1 , using machine automatic spotting MA antigen solution, CAP antigen solution, PCN antigen solution, SAS antigen solution, GM antigen solution, TC antigen solution concentration is 0.05mg / mL, each spot 20nL, the spot distribution of each antibody is as follows figure 1 shown.

[0042] (3) closed process;

[0043] The spotted black slide was submerged in blocking soluti...

Embodiment 2

[0047] A protein chip for the detection of veterinary drug residues, the preparation method of the protein chip comprises the following steps:

[0048] (1) Black slide pretreatment;

[0049] ① Soak the black slide in the slide pretreatment solution containing 2% NaOH for 24 hours, and then wash it with purified water for 2 to 8 times;

[0050] ② Soak black slides in 0.5% silane solution (medium: 25% ethanol) for 30 minutes;

[0051] ③Purge the soaked black glass slides with nitrogen gas, put them in an oven, and bake them at 140°C for 0.5h.

[0052] (2) Antigen solution spotting;

[0053] refer to figure 1 , use machine automation to spot MA antigen solution, CAP antigen solution, PCN antigen solution, SAS antigen solution, GM antigen solution, the concentration of antigen solution is 0.05mg / mL, and each spot spot is 20nL. The spot distribution of each antibody is as follows: figure 1 shown.

[0054] (3) closed process;

[0055] The spotted black slide was submerged in b...

Embodiment 3

[0059] A protein chip for the detection of veterinary drug residues, the preparation method of the protein chip comprises the following steps:

[0060] (1) Black slide pretreatment;

[0061] ① Soak the black slide in the slide pretreatment solution containing 2% NaOH for 20 hours, and then wash it with purified water for 2 to 8 times;

[0062] ② Soak black slides in 1% silane solution (medium: 25% ethanol) for 20 minutes;

[0063] ③Purge the soaked black glass slides with nitrogen gas, put them into an oven, and bake them at 100°C for 0.6h.

[0064] (2) Antigen solution spotting;

[0065] refer to figure 1 , use machine automation to spot MA antigen solution, CAP antigen solution, PCN antigen solution, SAS antigen solution, GM antigen solution, the concentration of antigen solution is 0.05mg / mL, and each spot spot is 20nL. The spot distribution of each antibody is as follows: figure 1 shown.

[0066] (3) closed process;

[0067] The spotted black slide was submerged in b...

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PUM

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Abstract

The invention discloses a protein chip for determining the residual concentration of six veterinary drugs in milk. A preparation method of the protein chip comprises the following steps of (1) pretreatment of black slide, (2) sample application of an antigen solution, and (3) blocking process performing and the protein chip preparation. The protein chip is used for detecting the residues of veterinary drugs in the milk, joint detection is realized by using an original protein chip technology of the company, the detection efficiency can be effectively improved, and the detection cost is reduced; and the inventor develops a protein chip diagnostic kit of six indexes of MA, CAP, PCN, SAS, GM and TC, and the advantages of speediness, high efficiency, low cost and the like are achieved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a protein chip for detecting veterinary drug residues and a preparation method thereof. Background technique [0002] Veterinary drug residues refer to the accumulation or retention of the original drug or its metabolites in the body of livestock or poultry or in the product after drug administration. The correct and reasonable use of veterinary drugs has brought high benefits to the breeding industry, but there are always some people who blindly pursue economic interests and illegally use, abuse, add or inject veterinary drugs or harmful substances in all aspects of the livestock and poultry industry. The residues and harmful substances of veterinary drugs seriously affect the safety of animal food and the sustainable development of animal husbandry, and more seriously, they directly endanger human health and life safety, and are extremely harmful to human health. Therefore, animal...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/552G01N33/531
CPCG01N33/531G01N33/552G01N33/6803
Inventor 邓仲锦泮锋纲丁俊杰施启尧
Owner 江苏三联生物工程股份有限公司
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